ArticleAllergy2021
Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity.
Article in Allergy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Advances in clinical examination techniques for allergic rhinitis: a review from traditional testing to precision diagnosis.Frontiers in allergy · 2026Review
- A novel oral immunotherapy strategy using transgenic barley inducesFrontiers in immunology · 2026Article
- Early allergen introduction overrides allergy predisposition in offspring of horses withFrontiers in immunology · 2025Article
- Article
- Allergen immunotherapy using recombinantFrontiers in allergy · 2024Article
- Comparison of Skin Prick Tests (SPT), Intradermal Tests (IDT) andAnimals : an open access journal from MDPI · 2023Article
- Insect Bite Hypersensitivity in Horses: Causes, Diagnosis, Scoring and New Therapies.Animals : an open access journal from MDPI · 2023Review
- The Effect of Insect Bite Hypersensitivity on Movement Activity and Behaviour of the Horse.Animals : an open access journal from MDPI · 2023Article
- Immunoproteomics reveal increased serum IgG3/5 binding toFrontiers in immunology · 2023Article
- Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma.Journal of veterinary internal medicine · 2023Article
- Equine keratinocytes in the pathogenesis of insect bite hypersensitivity: Just another brick in the wall?PloS one · 2022Article
- Article
- First clinical expression of equine insect bite hypersensitivity is associated with co-sensitization to multiple Culicoides allergens.PloS one · 2021Article
- Microarray molecular mapping of horses with severe asthma.Journal of veterinary internal medicineArticle
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Authors and funding
14 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAllergy to bites of blood-sucking insects, including biting midges, can affect both human and veterinary patients. Horses are often suffering from an IgE-mediated allergic dermatitis caused by bites of midges (Culicoides spp). With the aim to improve allergen immunotherapy (AIT), numerous Culicoides allergens have been produced as recombinant (r-) proteins. This study aimed to test a comprehensive panel of differently expressed Culicoides r-allergens on a cohort of IBH-affected and control horses using an allergen microarray.
methodsIgE levels to 27 Culicoides r-allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut-offs selected using a specificity of 95% and seropositivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r-allergens giving the best performing test was determined using logistic regression analysis.
resultsSeropositivity was significantly higher in IBH horses compared with controls for 25 r-allergens. Nine Culicoides r-allergens were major allergens for IBH with seven of them binding IgE in sera from > 70% of the IBH-affected horses. Combination of these top seven r-allergens could diagnose > 90% of IBH-affected horses with a specificity of > 95%. Correlation between differently expressed r-allergens was usually high (mean = 0.69, range: 0.28-0.91).
conclusionThis microarray will be a powerful tool for the development of component-resolved, patient-tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species.
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