ArticleeLife2020
Building the vertebrate codex using the gene breaking protein trap library.
Open access · goldAbstract read
In one paragraph
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
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- A Primer Genetic Toolkit for Exploring Mitochondrial Biology and Disease Using Zebrafish.Genes · 2022Article
- Cre/eLife · 2022Article
- The FusX TALE Base Editor (FusXTBE) for Rapid Mitochondrial DNA Programming of Human CellsThe CRISPR journal · 2021Article
- Disruption ofBioMed research international · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
31 authors at 8 institutions in 5 countries.
Noriko IchinoDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0002-7009-8299
MaKayla R SerresDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Rhianna M UrbanDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0001-6399-5015
Mark D UrbanDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0002-9992-7820
Anthony J TreichelDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0002-4393-7034
Kyle J SchaefbauerDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Lauren E TallantDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Gaurav K VarshneyTranslational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, United States.ORCID 0000-0002-0429-1904
Kimberly J SkusterDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Melissa S McNultyDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Camden L DabyDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Ying WangDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.
Hsin-Kai LiaoDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.
Suzan El-RassZebrafish Centre for Advanced Drug Discovery & Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute, St. Michael's Hospital, Unity Health Toronto & University of Toronto, Toronto, Canada.ORCID 0000-0003-2075-4275
Yonghe DingDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Weibin LiuDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Jennifer L AndersonDepartment of Embryology, Carnegie Institution for Science, Baltimore, United States.
Mark D WishmanDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Ankit SabharwalDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0003-4355-0355
Lisa A SchimmentiDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.
Sridhar SivasubbuGenomics and Molecular Medicine Unit, CSIR-Institute of Genomics and Integrative Biology, Delhi, India.
Darius BalciunasDepartment of Biology, Temple University, Philadelphia, United States.ORCID 0000-0003-1938-3243
Matthias HammerschmidtInstitute of Zoology, Developmental Biology Unit, University of Cologne, Cologne, Germany.ORCID 0000-0002-3709-8166
Steven Arthur FarberDepartment of Embryology, Carnegie Institution for Science, Baltimore, United States.ORCID 0000-0002-8037-7312
Xiao-Yan WenZebrafish Centre for Advanced Drug Discovery & Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute, St. Michael's Hospital, Unity Health Toronto & University of Toronto, Toronto, Canada.
Xiaolei XuDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0002-4928-3422
Maura McGrailDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.ORCID 0000-0001-9308-6189
Jeffrey J EssnerDepartment of Genetics, Development and Cell Biology, Iowa State University, Ames, United States.ORCID 0000-0001-8816-3848
Shawn M BurgessTranslational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, United States.ORCID 0000-0003-1147-0596
Karl J ClarkDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0002-9637-0967
Stephen C EkkerDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States.ORCID 0000-0003-0726-4212
WinnMed · USIowa State University · USCarnegie Institution for Science · USNational Institutes of Health · USSt. Michael's Hospital · CAInstitute of Genomics and Integrative Biology · INTemple University · USUniversity of Cologne · DE
Funding
Zebrafish targeted mutagenesis and functional genomicsZIAHG000183 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI BURGESS, SHAWN M · 2009 to 2025
$16.7MSystematic Vertebrate Functional GenomicsR01GM063904 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EKKER, STEPHEN CARL · 2001 to 2025
$10.7MGenetic Studies of Sarcomere-based Cardiac Diseases (Diversity Supplement)R01HL081753 · NHLBI · MAYO CLINIC ROCHESTER · PI XU, XIAOLEI · 2005 to 2025
$7.8MIdentifying genes required for digestive physiology and lipid metabolismR01DK093399 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI STEVEN A FARBER, John F Rawls · 2013 to 2026
$7.6MEpicardial remodeling in cardiomyopathy and cardiac agingR01HL107304 · NHLBI · MAYO CLINIC ROCHESTER · PI Xiaolei Xu · 2011 to 2026
$6.6MIntron-based Mutagenic Transposons for ZebrafishR01DA014546 · NIDA · UNIVERSITY OF MINNESOTA TWIN CITIES · PI EKKER, STEPHEN CARL · 2001 to 2011
$3.5MInternational Zebrafish Mutagenic Protein TrapR01HG006431 · NHGRI · MAYO CLINIC ROCHESTER · PI EKKER, STEPHEN CARL · 2011 to 2016
$3.3MSystematic Vertebrate Functional GenomicsR56GM063904 · NIGMS · MAYO CLINIC ROCHESTER · PI EKKER, STEPHEN CARL · 2009 to 2010
$611kGenes required for lipid processing and digestive organ function in larval zebrafR56DK093399 · NIDDK · CARNEGIE INSTITUTION OF WASHINGTON, D.C. · PI FARBER, STEVEN A · 2011 to 2011
$191kCouncil of Scientific and Industrial Research MLP1801Intramural NIH HHS ZIA HG000183Natural Sciences and Engineering Research Council of Canada RGPIN 05389-14NHGRI NIH HHS R01 HG006431NHLBI NIH HHS R01 HL081753NHLBI NIH HHS R01 HL107304NIDA NIH HHS R01 DA014546NIDDK NIH HHS R01 DK093399NIDDK NIH HHS R56 DK093399NIGMS NIH HHS R01 GM063904NIGMS NIH HHS R56 GM063904NIH HHS DA14546NIH HHS DK093399NIH HHS GM63904NIH HHS HG006431The intramural Reserch Program of the National Human Genome Research Institute, National Institutes of Health 1ZIAHG000183The Roy J. Carver Charitable Trust 07-2991
Abstract
One key bottleneck in understanding the human genome is the relative under-characterization of 90% of protein coding regions. We report a collection of 1200 transgenic zebrafish strains made with the gene-break transposon (GBT) protein trap to simultaneously report and reversibly knockdown the tagged genes. Protein trap-associated mRFP expression shows previously undocumented expression of 35% and 90% of cloned genes at 2 and 4 days post-fertilization, respectively. Further, investigated alleles regularly show 99% gene-specific mRNA knockdown. Homozygous GBT animals in
Indexed as
Gene Knockdown TechniquesGene LibraryGenes, ReporterAnimalsAnimals, Genetically ModifiedRNA, MessengerZebrafishZebrafish ProteinsRNA, MessengerZebrafish Proteinsdevelopmental biologydisease modelgene-break transposongene reversionhuman genetic disorderslight sheet microscopyprotein trapzebrafish
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.