A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr).

Katsuya Iuchi, Yasushi Arai, Kazuki Sasaki, Naoe Sato, Chikako Yokoyama, Toshiro Saruwatari, Hisashi Hisatomi

Open access · bronzeAbstract read

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Article in Cytotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 44% of its field

1 · What the graph read from it

What it found

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1 citing paper in PubMed, 3 citations in OpenAlex.

Estrogenic Effect of Various Plant Extracts on Eel (Molecules (Basel, Switzerland) · 2025
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4 · The record

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5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Katsuya IuchiDepartment of Materials and Life Science, Faculty of Science and Technology, Seikei University, 3-3-1 Kichijojikitamachi, Musashino-shi, Tokyo, 180-8633, Japan. iuchi@st.seikei.ac.jp.ORCID http://orcid.org/0000-0003-0625-6018

Yasushi AraiSeikei Junior and Senior High School, 3-10-13 Kichijojikitamachi, Musashino-shi, Tokyo, 180-8633, Japan.

Kazuki SasakiDepartment of Materials and Life Science, Faculty of Science and Technology, Seikei University, 3-3-1 Kichijojikitamachi, Musashino-shi, Tokyo, 180-8633, Japan.

Naoe SatoSeikei Junior and Senior High School, 3-10-13 Kichijojikitamachi, Musashino-shi, Tokyo, 180-8633, Japan.

Chikako YokoyamaDepartment of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan.

Toshiro SaruwatariAtmosphere and Ocean Research Institute, The Univeristy of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8564, Japan.

Hisashi HisatomiDepartment of Materials and Life Science, Faculty of Science and Technology, Seikei University, 3-3-1 Kichijojikitamachi, Musashino-shi, Tokyo, 180-8633, Japan.

Seikei University · JPYamagata University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

White-spotted charr (Salvelinus leucomaenis, S. I.) is an anadromous cold water-adapted fish, distributed in the Far East. We have previously reported the complete mitochondrial DNA sequences of white-spotted chars (S. l. imbrius and S. l. pluvius) in Japan. In general, fish hepatocytes are useful for cellular and biochemical studies of fish. In this study, we isolated hepatocytes from the liver of white-spotted charr and used basic methods, such as enzyme digestion and low centrifugation, to analyze the molecular mechanisms involved in specific cellular responses. The isolated hepatocytes could be cultured at 5-20 °C but not 37 °C. The morphology of hepatocytes was altered in a temperature-dependent manner. The properties of hepatocyte were similar to those of living fish. Moreover, the proliferation rate and damage of isolated hepatocytes depended on the concentration of fetal bovine serum in the culture medium. Taken together, this study demonstrates that this simple method for isolation and culture of hepatocytes from white-spotted charr may be useful for other biochemical and cellular studies.

Indexed as

Cell cultureExperimental modelHepatocytesWhite-spotted charr

Identifiers

PMID32779071

DOI10.1007/s10616-020-00415-6

PMCPMC7547923

OpenAlexW3048427149

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.