Evidence map›Paper›PMID 32778838›Full record

ArticleNature protocols2020

Direct analysis of brain phenotypes via neural blastocyst complementation.

Hai-Qiang Dai, Zhuoyi Liang, Amelia N Chang, Aimee M Chapdelaine-Williams, Beatriz Alvarado, Alex A Pollen, Frederick W Alt, Bjoern Schwer

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Hai-Qiang Dai *Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Genetics, and Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
Zhuoyi Liang *Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Genetics, and Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
Amelia N Chang *Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Genetics, and Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
Aimee M Chapdelaine-WilliamsHoward Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Genetics, and Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
Beatriz AlvaradoDevelopmental and Stem Cell Biology Graduate Program, University of California, San Francisco, San Francisco, CA, USA.
Alex A PollenDevelopmental and Stem Cell Biology Graduate Program, University of California, San Francisco, San Francisco, CA, USA.
Frederick W AltHoward Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Department of Genetics, and Department of Pediatrics, Harvard Medical School, Boston, MA, USA. alt@enders.tch.harvard.edu.ORCID http://orcid.org/0000-0002-0583-1271
Bjoern SchwerDevelopmental and Stem Cell Biology Graduate Program, University of California, San Francisco, San Francisco, CA, USA. bjoern.schwer@ucsf.edu.ORCID http://orcid.org/0000-0003-4367-6265
Boston Children's Hospital · USUniversity of California, San Francisco · US

Funding

Mechanisms that Control IgH Variable Region Exon AssemblyR01AI020047 · NIAID · IMMUNE DISEASE INSTITUTE, INC. · PI ALT, FREDERICK W. · 1985 to 2025
$14.1M
PREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7M
Role of DNA double-strand breaks in neural function and homeostasisR56AG064363 · NIA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI POSKANZER, KIRA, SCHWER, BJOERN · 2019 to 2019
$402k
Howard Hughes Medical InstituteNIAID NIH HHS R01 AI020047NIA NIH HHS R56 AG064363NICHD NIH HHS T32 HD007470
6 · The paper itself

Abstract

We provide a protocol for generating forebrain structures in vivo from mouse embryonic stem cells (ESCs) via neural blastocyst complementation (NBC). We developed this protocol for studies of development and function of specific forebrain regions, including the cerebral cortex and hippocampus. We describe a complete workflow, from methods for modifying a given genomic locus in ESCs via CRISPR-Cas9-mediated editing to the generation of mouse chimeras with ESC-reconstituted forebrain regions that can be directly analyzed. The procedure begins with genetic editing of mouse ESCs via CRISPR-Cas9, which can be accomplished in ~4-8 weeks. We provide protocols to achieve fluorescent labeling of ESCs in ~2-3 weeks, which allows tracing of the injected, ESC-derived donor cells in chimeras generated via NBC. Once modified ESCs are ready, NBC chimeras are generated in ~3 weeks via injection of ESCs into genetically programmed blastocysts that are subsequently transferred into pseudo-pregnant fosters. Our in vivo brain organogenesis platform is efficient, allowing functional and systematic analysis of genes and other genomic factors in as little as 3 months, in the context of a whole organism.

Indexed as

AnimalsBlastocystBrainBrain MappingCell DifferentiationChimeraFemaleMaleMiceMouse Embryonic Stem CellsOrganogenesisPhenotype

Identifiers

PMID32778838
PMCPMC7685531
OpenAlexW3048350454

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.