ArticleNature methods2020
Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Nucleophagy is promoted by two autophagy receptors and inhibited by chromatin-nuclear envelope tethering in fission yeast.Nature communications · 2026Article
- Cloneable contrast across all biological length scales.Journal of structural biology · 2026Article
- Molecular tags for electron cryo-tomography.Emerging topics in life sciences · 2025Review
- SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials.Journal of nanobiotechnology · 2025Review
- Review
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast.PLoS biology · 2023Article
- Single probes and resonant four-wave-mixing enabling novel correlative light electron microscopy workflow.Light, science & applications · 2023Article
- A Convenient All-Cell Optical Imaging Method Compatible with Serial SEM for Brain Mapping.Brain sciences · 2023Article
- Correlative light-electron microscopy using small gold nanoparticles as single probes.Light, science & applications · 2023Article
- In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview.Sub-cellular biochemistry · 2023Review
- A Validated Set of Ascorbate Peroxidase-Based Organelle Markers for Electron Microscopy of Saccharomyces cerevisiae.mSphere · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Gold labelling of a green fluorescent protein (GFP)-tag inside cells using recombinant nanobodies conjugated to 2.4 nm thiolate-coated gold nanoparticles.Nanoscale advances · 2021Article
- Reagents and models for detecting endogenous GLP1R and GIPR.EBioMedicine · 2021Review
- Engineered Repeat Protein Hybrids: The New Horizon for Biologic Medicines and Diagnostic Tools.Accounts of chemical research · 2021Article
- Niwaki Instead of Random Forests: Targeted Serial Sectioning Scanning Electron Microscopy With Reimaging Capabilities for Exploring Central Nervous System Cell Biology and Pathology.Frontiers in neuroanatomy · 2021Review
- Three-Dimensional Structure of Dendritic Spines Revealed by Volume Electron Microscopy Techniques.Frontiers in neuroanatomy · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically encoded tags for single-molecule imaging in electron microscopy (EM) are long-awaited. Here, we report an approach for directly synthesizing EM-visible gold nanoparticles (AuNPs) on cysteine-rich tags for single-molecule visualization in cells. We first uncovered an auto-nucleation suppression mechanism that allows specific synthesis of AuNPs on isolated tags. Next, we exploited this mechanism to develop approaches for single-molecule detection of proteins in prokaryotic cells and achieved an unprecedented labeling efficiency. We then expanded it to more complicated eukaryotic cells and successfully detected the proteins targeted to various organelles, including the membranes of endoplasmic reticulum (ER) and nuclear envelope, ER lumen, nuclear pores, spindle pole bodies and mitochondrial matrices. We further implemented cysteine-rich tag-antibody fusion proteins as new immuno-EM probes. Thus, our approaches should allow biologists to address a wide range of biological questions at the single-molecule level in cellular ultrastructural contexts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.