ArticleScientific reports2020
Karyotype changes in long-term cultured tick cell lines.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Cultivation ofJournal of veterinary research · 2025Article
- Use of Tick Cell Lines in Co-Infection Studies with a Preliminary Study of Co-Culture ofPathogens (Basel, Switzerland) · 2025Article
- Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line.Computational and structural biotechnology journal · 2025Article
- The highly improved genome ofLife science alliance · 2023Article
- New Cell Lines Derived from European Tick Species.Microorganisms · 2022Article
- Article
- Article
- Universal fluorescence in situ hybridization (FISH) protocol for mapping repetitive DNAs in insects and other arthropods.Molecular genetics and genomics : MGG · 2021Article
- How relevant are in vitro culture models for study of tick-pathogen interactions?Pathogens and global healthReview
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
Abstract
Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n = 28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n = 20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account.
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Registered trials
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