Evidence map›Paper›PMID 32778248›Full record

ArticleActa histochemica2020

The validation of the Helicobacter pylori CagA typing by immunohistochemistry: nationwide application in Indonesia.

Muhammad Miftahussurur, Dalla Doohan, Ari Fahrial Syam, Iswan Abbas Nusi, Langgeng Agung Waskito, Kartika Afrida Fauzia, Yudith Annisa Ayu Rezkitha, Astri Dewayani, Reny I'tishom, Hasan Maulahela and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta histochemica, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Update on the pathogenesis and clinical management ofWorld journal of gastrointestinal pathophysiology · 2025
    Review
  3. Frontiers in microbiology · 2024
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 3 countries.

Muhammad MiftahussururGastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, 60131, Indonesia; Institute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia. Electronic address: muhammad-m@fk.unair.ac.id.
Dalla DoohanInstitute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, 879-5593, Japan.
Ari Fahrial SyamDivision of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, 16424, Indonesia.
Iswan Abbas NusiGastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, 60131, Indonesia.
Langgeng Agung WaskitoInstitute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, 879-5593, Japan.
Kartika Afrida FauziaInstitute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, 879-5593, Japan.
Yudith Annisa Ayu RezkithaInstitute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia; Faculty of Medicine, University of Muhammadiyah Surabaya, Surabaya, 60113, Indonesia.
Astri DewayaniDepartment of Anatomy and Histology, Faculty of Medicine, Universitas Airlangga, Surabaya, 60131, Indonesia; Department of Infectious Disease, Oita University Faculty of Medicine, Yufu, 879-5593, Japan.
Reny I'tishomInstitute of Tropical Disease, Universitas Airlangga, Surabaya, 60115, Indonesia.
Hasan MaulahelaDivision of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, 16424, Indonesia.
Tomohisa UchidaDepartment of Molecular Pathology, Oita University Faculty of Medicine, Yufu, 879-5593, Japan.
Yoshio YamaokaGastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, 60131, Indonesia; Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, 879-5593, Japan; Department of Gastroenterology and Hepatology, Baylor College of Medicine and Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX, 77030, USA; Global Oita Medical Advanced Research Center for Health (GO-MARCH), Yufu, 870-1192, Japan. Electronic address: yyamaoka@oita-u.ac.jp.
Airlangga University · IDOita University · JPUniversity of Indonesia · IDMichael E. DeBakey VA Medical Center · USUniversitas Muhammadiyah Jember · ID

Funding

REGULATION OF CXC CHEMOKINE EXPRESSION BY H. PYLORIR01DK062813 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI YAMAOKA, YOSHIO · 2004 to 2013
$3.0M
NIDDK NIH HHS R01 DK062813
6 · The paper itself

Abstract

We aimed to validate 2 types of antibodies, anti-CagA antibody and anti-East Asian CagA specific antibody (α-EAS antibody) for the determination of CagA status in Indonesia. We also confirmed the performance of α-EAS antibody for the detection of East Asian-type CagA H. pylori. Immunohistochemistry was performed using anti-CagA antibody and α-EAS antibody on gastric biopsy specimens from a total of 967 Indonesian patients. Diagnostic values of immunohistochemistry were evaluated with PCR-based sequencing as gold standard. Anti-CagA antibody had high sensitivity, specificity, and accuracy (87.0 %, 100 %, and 98.8 %, respectively) for determining CagA status. The α-EAS antibody was not suitable for the purpose of CagA status determination, as it had a low sensitivity (23.9 %). High specificity (97.6 %) but low sensitivity (41.2 %) and accuracy (66.3 %) was observed in α-EAS antibody to detect East Asian-type CagA. Patients with positive result of immunohistochemistry using anti-CagA antibody had significantly higher monocyte infiltration score in antrum (P < 0.001) and corpus (P = 0.009). In conclusion, the anti-CagA antibody is still suitable to be used in Indonesia for determining the CagA status, whilst the α-EAS antibody was not appropriate to discriminate between East Asian-type and non-East Asian-type CagA in Indonesia.

Indexed as

Antibodies, BacterialAntigens, BacterialGastric MucosaHelicobacter pyloriHumansImmunohistochemistryIndonesiaAntibodies, BacterialAntigens, Bacterialanti-CagA antibodyAnti-East Asian CagA antibodyCagA typingImmunohistochemistryInfectious disease

Identifiers

PMID32778248
PMCPMC7486129
OpenAlexW3044158116

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.