Evidence map›Paper›PMID 32778115›Full record

ArticleBMC veterinary research2020

Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells.

Sonja Prpar Mihevc, Vesna Kokondoska Grgich, Andreja Nataša Kopitar, Luka Mohorič, Gregor Majdič

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sonja Prpar MihevcVeterinary Faculty, Institute of Preclinical Sciences, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia.
Vesna Kokondoska GrgichVeterinary Faculty, Institute of Preclinical Sciences, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia.
Andreja Nataša KopitarFaculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Zaloška 4, 1000, Ljubljana, Slovenia.
Luka MohoričAnimacel Ltd, Mivka 34, 1000, Ljubljana, Slovenia.
Gregor MajdičVeterinary Faculty, Institute of Preclinical Sciences, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia. gregor.majdic@vf.uni-lj.si.
University of Ljubljana · SI

Funding

European Social Fund Grant for SPMJavna Agencija za Raziskovalno Dejavnost RS P4-0053
6 · The paper itself

Abstract

backgroundThe ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine, especially for replacing neuronal tissue damaged by different neurological disorders. Reprogramming of ASCs can be induced by the growth medium with neurogenic inductors and specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement, and retinoic acid. Cells were first preconditioned in the pre-differentiation neural induction medium (mitogenically stimulated; STIM1), followed by the induction of neuronal differentiation.

resultsAfter 3, 6, and 9 days of neural induction, elongated neural-like cells with bipolar elongations were observed, and some oval cells with light nuclei appeared. The expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H), microtubule-associated protein-2 (MAP2), and glial fibrillary acidic protein (GFAP) was observed using immunocytochemistry, which confirmed the differentiation into neurons and glial cells. Flow cytometry analysis showed high GFAP expression (between 70 and 90% of all cells) after cells had been growing three days in the neural induction medium a (NIMa). Around 25% of all cells also expressed adult neuronal markers NF-H and MAP2. After nine days of ASCs differentiation, the expression of all neural markers was reduced. There were no differences between the neural differentiation of ASCs isolated from female or male dogs.

conclusionsThe differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium, the canine ASCs differentiated into neural lineages and expressed markers of neuronal and glial cells, and also displayed the typical neuronal morphology. Differentiated ASCs can thus be a source of neural cellular lineages for the regenerative therapy of nerve damage and could be useful in the future for therapy or the modelling of neurodegenerative diseases.

Indexed as

Adipose TissueAnimalsCell DifferentiationCells, CulturedCulture MediaDogsFemaleMaleMesenchymal Stem CellsNeurogliaNeuronsCulture Mediaastrocytesdifferentiationdogmesenchymal stem cellsmultipotent mesenchymal stromal cellsneurons

Identifiers

PMID32778115
PMCPMC7418429
OpenAlexW3048651871

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.