ArticleMicrobial cell factories2020
Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 37 citations in OpenAlex.
- Multistep metabolic engineeredSynthetic and systems biotechnology · 2026Article
- Mixed sugars to a multipurpose chemical: perspectives of 2,3-butanediol fermentability in complex lignocellulose hydrolysates.Bioprocess and biosystems engineering · 2026Review
- Valorising Whey: From Environmental Burden to Bio-Based Production of Value-Added Compounds and Food Ingredients.Foods (Basel, Switzerland) · 2025Review
- Mixed Culture Cultivation in Microbial Bioprocesses.Advances in biochemical engineering/biotechnology · 2025Review
- Carrot Discard as a Promising Feedstock to Produce 2,3-Butanediol by Fermentation withBioengineering (Basel, Switzerland) · 2023Article
- Past, Present, and Future Perspectives on Whey as a Promising Feedstock for Bioethanol Production by Yeast.Journal of fungi (Basel, Switzerland) · 2022Review
- Non-Sterilized Fermentation of 2,3-Butanediol with Seawater by Metabolic Engineered Fast-GrowingFrontiers in bioengineering and biotechnology · 2022Article
- Engineering for an HPV 9-valent vaccine candidate using genomic constitutive over-expression and low lipopolysaccharide levels in Escherichia coli cells.Microbial cell factories · 2021Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundWhey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical.
resultsKlebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox, pta, frdA, ldhD, and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fed-batch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g.
conclusionThis study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.
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