Evidence map›Paper›PMID 32778107›Full record

ArticleBMC veterinary research2020

A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum.

Jan Plut, Urska Jamnikar-Ciglenecki, Irena Golinar-Oven, Tanja Knific, Marina Stukelj

Abstract read
In one paragraph

Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Porcine circovirus 3: a new challenge to explore.Frontiers in veterinary science · 2023
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jan PlutClinic for Ruminants and Pigs, Clinic for Reproduction and Farm Animals, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia. jan.plut@vf.uni-lj.si.ORCID http://orcid.org/0000-0003-3456-7438
Urska Jamnikar-CigleneckiDepartment of Food Safety, Institute of Safe Food, Animal Nutrition and Environment, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
Irena Golinar-OvenClinic for Ruminants and Pigs, Clinic for Reproduction and Farm Animals, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
Tanja KnificInstitute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
Marina StukeljClinic for Ruminants and Pigs, Clinic for Reproduction and Farm Animals, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPorcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences.

resultsPositive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups.

conclusionsThe results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.

Indexed as

AnimalsCircoviridae InfectionsCircovirusDNA, ViralFecesPhylogenyReal-Time Polymerase Chain ReactionSalivaSloveniaSwineSwine DiseasesViral VaccinesDNA, ViralViral VaccinesFaecesFirst reportPCRPCV3Pig oral fluidSerumSloveniaViral disease

Identifiers

PMID32778107
PMCPMC7419202

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.