Evidence map›Paper›PMID 32777951›Full record

ArticleBioTechniques2020

High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction.

Yuan Wen, Ivan J Vechetti, Taylor R Valentino, John J McCarthy

Open access · goldAbstract read
In one paragraph

Article in BioTechniques, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
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  4. Article
  5. β-glucan nanoparticles alleviate acute asthma by suppressing ferroptosis and DNA damage in mice.Apoptosis : an international journal on programmed cell death · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Host autophagy limitsFrontiers in microbiology · 2022
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Yuan WenDepartment of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.ORCID 0000-0002-3210-1629
Ivan J VechettiDepartment of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.
Taylor R ValentinoDepartment of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.
John J McCarthyDepartment of Physiology, College of Medicine, University of Kentucky, Lexington, KY, USA.
University of Kentucky · US

Funding

The role of satellite cells in adult skeletal muscle growth and maintenanceR01AR060701 · NIAMS · UNIVERSITY OF KENTUCKY · PI MCCARTHY, JOHN JOSEPH, PETERSON, CHARLOTTE A. · 2010 to 2019
$3.3M
NIAMS NIH HHS R01 AR060701
6 · The paper itself

Abstract

Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol

Indexed as

Blotting, WesternDNAGenomicsGuanidinesHumansMuscle ProteinsMuscle, SkeletalPhenolsRNADNAGuanidinesMuscle ProteinsPhenolsRNAtrizolethanol–bromochloropropane–water methodguanidinium thiocyanate–phenol–chloroform extractionmodified TRIzol protein isolationskeletal muscle

Identifiers

PMID32777951
PMCPMC7566772
OpenAlexW3045313182

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.