ArticleAnimal bioscience2021
In vitro ruminal fermentation of fenugreek (Trigonella foenum-graecum L.) produced less methane than that of alfalfa (Medicago sativa).
Article in Animal bioscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Fenugreek seed meal as a partial replacement for soybean meal: Dose-dependent effects on in vitro rumen fermentation and greenhouse gas production.Scientific reports · 2026Article
- Effect of different levels of Fenugreek sprouts on rumen microbiota and milk yield in Hassani goats.Scientific reports · 2026Article
- Effectiveness of second-gradeIranian journal of veterinary research · 2026Article
- Bioclimatic Influence on the Nutritional Composition, In Vitro Ruminal Fermentation Dynamics, and Greenhouse Gas Emissions ofAnimals : an open access journal from MDPI · 2025Article
- In silico docking and molecular dynamics for the discovery of inhibitors of enteric methane production in ruminants - A review.Animal bioscience · 2025Article
- Effects of herbal plant supplementation on rumen fermentation profiles and protozoan populationVeterinary world · 2024Article
- Application of fenugreek in ruminant feed: implications for methane emissions and productivity.PeerJ · 2024Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
objectiveThe objective of this study was to compare fenugreek (FG) with alfalfa (Alf) in ruminal fermentation and methane (CH4) production in vitro.
methodsWhole-plant FG harvested at 11- and 15-wk and Alf harvested at early and mid-bloom maturities, alone or as 50:50 mixture of FG and Alf at the respective maturity, were assessed in a series of 48-h in vitro batch culture incubations. Total fermentation gas and methane gas production, dry matter (DM) disappearance, volatile fatty acids, microbial protein and 16S RNA gene copy numbers of total bacteria and methanogens were determined.
resultsCompared to early bloom Alf, FG harvested at 11-wk exhibited higher (p<0.05) in vitro DM and neutral detergent fibre disappearance, but this difference was not observed between the mid-bloom Alf and 15-wk FG. Regardless plant maturity, in vitro ruminal fermentation of FG produced less (p<0.001) CH4 either on DM incubated or on DM disappeared basis than that of Alf during 48-h incubation. In vitro ruminal fermentation of FG yielded similar amount of total volatile fatty acids with higher (p<0.05) propionate percentage as compared to fermentation of Alf irrespective of plant maturity. Microbial protein synthesis was greater (p<0.001) with 11-wk FG than early bloom Alf as substrate and 16S RNA gene copies of total bacteria was higher (p<0.01) with 15-wk FG than mid-bloom Alf as substrate. Compared to mid-bloom Alf, 15-wk FG had lower (p<0.05 to 0.001) amount of 16S RNA methanogen gene copies in the whole culture during 48-h incubation.
conclusionIn comparison to Alf, FG emerges as a high quality forage that can not only improve rumen fermentation in vitro, but can also remarkably mitigate CH4 emissions likely due to being rich in saponins.
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