ArticleJournal of experimental botany2020
Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Metabolic engineering strategies for optimized lignan production in plants.Frontiers in plant science · 2026Review
- Physiological roles of lignins - tuning cell wall hygroscopy and biomechanics.The New phytologist · 2025Review
- Modulation of lignin and anthocyanin homeostasis by GTP cyclohydrolase1 in maize.Plant biotechnology journal · 2025Article
- Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid.Plant physiology · 2024Article
- Grass lignin: biosynthesis, biological roles, and industrial applications.Frontiers in plant science · 2024Review
- The impact of xylan on the biosynthesis and structure of extracellular lignin produced by a Norway spruce tissue culture.Plant direct · 2023Article
- Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis.The Plant cell · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Monolignol export by diffusion down a polymerization-induced concentration gradient.The Plant cell · 2022Article
- UGT72, a Major Glycosyltransferase Family for Flavonoid and Monolignol Homeostasis in Plants.Biology · 2022Review
- Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo.Plants (Basel, Switzerland) · 2021Article
- Mini Review: Transport of Hydrophobic Polymers Into the Plant Apoplast.Frontiers in plant science · 2020Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both the mechanisms of monolignol transport and the transported form of monolignols in developing xylem of trees are unknown. We tested the hypothesis of an active, plasma membrane-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from developing xylem and lignin-forming tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as from control materials, comprising non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested that this transport was through the tonoplast. Membrane vesicles prepared from lignin-forming spruce cells showed coniferin transport, but the Km value for coniferin was much higher than those of xylem and BY-2 cells. Liquid chromatography-mass spectrometry analysis of membrane proteins isolated from spruce developing xylem, phloem, and lignin-forming cultured cells revealed multiple transporters. These were compared with a transporter gene set obtained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for ABC-transporter-mediated monolignol transport but point to a role for secondary active transporters (such as MFS or MATE transporters). In contrast, proteomic and co-expression analyses suggested a role for ABC transporters and MFS transporters.
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