ArticlePLoS pathogens2020
JNK pathway restricts DENV2, ZIKV and CHIKV infection by activating complement and apoptosis in mosquito salivary glands.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 74 citations in OpenAlex.
- Research progress on the role of host factors in Chikungunya virus infection and intervention strategies.Virulence · 2026Review
- Development of a Novel Immune Gene Array to Assess Mosquito Gene Expression During Arbovirus Infection.Viruses · 2026Article
- Altered histone modifications in Aedes aegypti midguts following Rift Valley fever virus exposure.Scientific reports · 2026Article
- Review
- From gatekeeper to target: MAPK cascades as control circuits at the insect-microbe interface.FEMS microbiology reviews · 2026Review
- Entomological Surveillance and Vector Competence: A Crucial Front in Dengue Prevention.Current topics in microbiology and immunology · 2026Review
- Effects of Dominant Associated BacteriaMicroorganisms · 2025Article
- Thioester-Containing Protein TEP27 inInternational journal of molecular sciences · 2025Article
- Altered histone modifications inbioRxiv : the preprint server for biology · 2025Article
- Article
- From perception to action: MAPKs in plant-virus interactions.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The Hsf1-sHsp cascade has pan-antiviral activity in mosquito cells.Communications biology · 2025Article
- Why the Jenner/Pasteur paradigm is insufficient for controlling vector-borne diseases and the role of microbiota-mediated interactions.Current research in parasitology & vector-borne diseases · 2025Review
- UnveilingFrontiers in cellular and infection microbiology · 2025Article
- Peptides with Antimicrobial Activity in the Saliva of the Malaria VectorInternational journal of molecular sciences · 2024Article
- Effect of C-type lectin 16 on dengue virus infection inPNAS nexus · 2024Article
- Immune Gene Repertoire of Soft Scale Insects (Hemiptera: Coccidae).International journal of molecular sciences · 2024Article
- Evaluation of vector susceptibility inmSphere · 2024Article
- Immune Reactions of Vector Insects to Parasites and Pathogens.Microorganisms · 2024Review
- Niemann-Pick Type C2 Proteins inInternational journal of molecular sciences · 2024Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arbovirus infection of Aedes aegypti salivary glands (SGs) determines transmission. However, there is a dearth of knowledge on SG immunity. Here, we characterized SG immune response to dengue, Zika and chikungunya viruses using high-throughput transcriptomics. We also describe a transcriptomic response associated to apoptosis, blood-feeding and lipid metabolism. The three viruses differentially regulate components of Toll, Immune deficiency (IMD) and c-Jun N- terminal Kinase (JNK) pathways. However, silencing of the Toll and IMD pathway components showed variable effects on SG infection by each virus. In contrast, regulation of the JNK pathway produced consistent responses in both SGs and midgut. Infection by the three viruses increased with depletion of the activator Kayak and decreased with depletion of the negative regulator Puckered. Virus-induced JNK pathway regulates the complement factor, Thioester containing protein-20 (TEP20), and the apoptosis activator, Dronc, in SGs. Individual and co-silencing of these genes demonstrate their antiviral effects and that both may function together. Co-silencing either TEP20 or Dronc with Puckered annihilates JNK pathway antiviral effect. Upon infection in SGs, TEP20 induces antimicrobial peptides (AMPs), while Dronc is required for apoptosis independently of TEP20. In conclusion, we revealed the broad antiviral function of JNK pathway in SGs and showed that it is mediated by a TEP20 complement and Dronc-induced apoptosis response. These results expand our understanding of the immune arsenal that blocks arbovirus transmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.