ArticleEnvironmental science and pollution research international2020
Detection of Prorocentrum minimum by hyperbranched rolling circle amplification coupled with lateral flow dipstick.
Article in Environmental science and pollution research international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
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- Review
- A Novel Sample-to-Answer Visual Nucleic Acid Detection System for Adenovirus Detection.Microbiology spectrum · 2023Article
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6 authors.
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Abstract
A novel method referred to as hyperbranched rolling circle amplification (HRCA) coupled with lateral flow dipstick (LFD) (HRCA-LFD) here was developed for specific, sensitive, rapid, and simple detection of Prorocentrum minimum. HRCA-LFD relies on a padlock probe (PLP) consisting of a common ligation sequence, two terminal sequences that complement the target DNA, and a manually designed detection probe (LFD probe). The two terminal sequences of the PLP were designed against the species-specific sites of the large subunit ribosomal DNA (LSU rDNA) D1-D2 region of P. minimum. The optimum parameters for HRCA were as follows: PLP concentration of 20 pM, ligation time of 30 min, ligation temperature of 59 °C, enzymic digestion time of 105 min, amplification time of 45 min, and amplification temperature of 58 °C. The HRCA-LFD displaying high specificity could accurately distinguish P. minimum from other microalgae. The detection limit of HRCA-LFD was as low as 1.42 × 10
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