ArticleBioprocess and biosystems engineering2021
Heterologous expression and biochemical characterization of a thermostable endo-β-1,4-glucanase from Colletotrichum orchidophilum.
Article in Bioprocess and biosystems engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 17 citations in OpenAlex.
- Low oxygen environment effect on the tomato cell wall composition during the fruit ripening process.BMC plant biology · 2024Article
- Production and Characterization of Cellulose Nanocrystals from Eucalyptus Dissolving Pulp Using Endoglucanases fromInternational journal of molecular sciences · 2023Article
- Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01.Scientific reports · 2022Article
- Heterologous expression and characterization of two novel glucanases derived from sheep rumen microbiota.World journal of microbiology & biotechnology · 2022Article
- Hydrolytic enzyme production from açai palm (Euterpe precatoria) endophytic fungi and characterization of the amylolytic and cellulolytic extracts.World journal of microbiology & biotechnology · 2022Article
- Development of synthetic biology tools to engineerSynthetic and systems biotechnology · 2021Review
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
To develop new cellulases for efficient utilization of the lignocellulose, an endoglucanase (CoCel5A) gene from Colletotrichum orchidophilum was synthesized and a recombinant Pichia pastoris GS115/pPIC9K/cocel5A was constructed for secretory expression of CoCel5A. After purification, the protein CoCel5A was biochemically characterized. The endoglucanase CoCel5A exhibited the optimal activity at 55-75 °C and high thermostability (about 85% residual activity) at the temperature of 55 °C after incubation for 3 h. The highest activity of CoCel5A was detected when 100 mM citric acid buffer (pH 4.0-5.0) was used and excellent pH stability (up to 95% residual activity) was observed after incubation in 100 mM citric acid buffer (pH 3.0-6.0) at 4 °C for 24 h. Carboxymethyl cellulose sodium salt (n = approx. 500) (CMC) and β-D-glucan were the best substrates for CoCel5A among the tested substrates. The kinetic parameters V
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Registered trials
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