Evidence map›Paper›PMID 32771055›Full record

ArticleStem cell research & therapy2020

Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson's disease.

Julian L Goggi, Lifeng Qiu, Mei Chih Liao, Shivashankar Khanapur, Lingfan Jiang, Ramasamy Boominathan, Siddesh V Hartimath, Peter Cheng, Fui Fong Yong, Vanessa Soh and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  6. PET reporter systems for the brain.Trends in neurosciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Julian L GoggiSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Lifeng QiuNeural Stem Cell Research Lab, Research Department, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore.
Mei Chih LiaoBioprocessing Technology Institute, A*STAR, 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Shivashankar KhanapurSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Lingfan JiangSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Ramasamy BoominathanSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Siddesh V HartimathSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Peter ChengSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Fui Fong YongSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Vanessa SohSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Xiaozhou DengSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Youshan Melissa LinBioprocessing Technology Institute, A*STAR, 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Anna HaslopSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Peng Wen TanSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Xiaoxia ZengNeural Stem Cell Research Lab, Research Department, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore.
Jolene W L LeeNeural Stem Cell Research Lab, Research Department, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore.
Zhiwei ZhangNeural Stem Cell Research Lab, Research Department, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore.
Pragalath SadasivamSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore.
Eng King TanResearch Department, National Neuroscience Institute, SGH Campus, Singapore, 169856, Singapore.
Sajinder K LuthraGE Healthcare Life Sciences, White Lion Rd., Little Chalfont, Amersham, HP7 9LL, UK.
William D ShingletonGE Healthcare Life Sciences, White Lion Rd., Little Chalfont, Amersham, HP7 9LL, UK.
Steve K W OhBioprocessing Technology Institute, A*STAR, 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore.
Li ZengNeural Stem Cell Research Lab, Research Department, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore. Li_Zeng@nni.com.sg.
Edward G RobinsSingapore Bioimaging Consortium, A*STAR, 11 Biopolis Way, #01-02 HELIOS, Singapore, 138667, Singapore. Edward_Robins@sbic.a-star.edu.sg.ORCID 0000-0002-0156-8082

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSignificant developments in stem cell therapy for Parkinson's disease (PD) have already been achieved; however, methods for reliable assessment of dopamine neuron maturation in vivo are lacking. Establishing the efficacy of new cellular therapies using non-invasive methodologies will be critical for future regulatory approval and application. The current study examines the utility of neuroimaging to characterise the in vivo maturation, innervation and functional dopamine release of transplanted human embryonic stem cell-derived midbrain dopaminergic neurons (hESC-mDAs) in a preclinical model of PD.

methodsFemale NIH RNu rats received a unilateral stereotaxic injection of 6-OHDA into the left medial forebrain bundle to create the PD lesion. hESC-mDA cell and sham transplantations were carried out 1 month post-lesion, with treated animals receiving approximately 4 × 10

resultsPET imaging revealed transplant survival and maturation into functional dopaminergic neurons. [

conclusionsThis study provides further evidence for the value of in vivo functional imaging for the assessment of cell therapies and highlights the utility of DAT imaging for the determination of early post-transplant cell maturation and differentiation of hESC-mDAs.

Indexed as

Dopaminergic NeuronsParkinson DiseasePositron Emission Tomography Computed TomographyAnimalsDisease Models, AnimalDopamine Plasma Membrane Transport ProteinsFemaleNeuroimagingOxidopamineRatsDopamine Plasma Membrane Transport ProteinsOxidopamineCell therapyDATDopaminergic neuronParkinson’s diseasePET imaging

Identifiers

PMID32771055
PMCPMC7414543

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.