ArticleJournal of orthopaedic surgery and research2020
Apolipoprotein D alleviates glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells via the PI3K/Akt pathway.
Article in Journal of orthopaedic surgery and research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- The Lipocalin Apolipoprotein D Functional Portrait: A Systematic Review.Frontiers in physiology · 2021Pooled it
- Comprehensive bioinformatics analysis reveals novel potential biomarkers associated with aging and mitochondria in osteoporosis.Scientific reports · 2025Article
- Osteogenic potential of Frondoside A in human periodontal ligament cells: an RNA-Seq analysis.Journal of periodontal & implant science · 2024Article
- Analysis of metastasis‑related risk factors and clinical relevance in adult soft‑tissue sarcoma.Oncology letters · 2024Article
- Article
- Bioinformatic analysis of related immune cell infiltration and key genes in the progression of osteonecrosis of the femoral head.Frontiers in immunology · 2023Article
- Regulation of Chondrocyte Differentiation by miR-455-3p Secreted by Bone Marrow Stem Cells through Phosphatase and Tensin Homolog Deleted on Chromosome Ten/Phosphoinositide 3-Kinase-Protein Kinase B.Stem cells international · 2023Article
- A novel serological biomarker are associated with disease severity in patients with osteoarthritis.Journal of bone and mineral metabolism · 2022Article
- Evaluation of candidate reference genes for quantitative real-time PCR normalization in blood from red deer developing antlers.Scientific reports · 2022Article
- The Neuroprotective Lipocalin Apolipoprotein D Stably Interacts with Specific Subtypes of Detergent-Resistant Membrane Domains in a Basigin-Independent Manner.Molecular neurobiology · 2022Article
- Differential circRNA and mRNA Expression Profiling During Osteogenic Differentiation in Rat Bone Marrow Mesenchymal Stem Cells.Medical science monitor : international medical journal of experimental and clinical research · 2022Article
- Preparation, characterization, and osteogenic activity mechanism of casein phosphopeptide-calcium chelate.Frontiers in nutrition · 2022Article
- Inhibition of miRNA-100 facilitates bone regeneration defects of mesenchymal stem cells in osteoporotic mice through the protein kinase B pathway.Bioengineered · 2022Article
- Enhanced Osteogenic Differentiation of Pluripotent Stem Cells via γ-Secretase Inhibition.International journal of molecular sciences · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundTo clarify the role of apolipoprotein D (Apod) in alleviating glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells (MSCs) via the PI3K/Akt pathway, thus influencing the progression of osteoporosis (OP).
methodsOsteogenesis in MSCs was induced by dexamethasone (DEX) stimulation. Dynamic expressions of Apod in MSCs undergoing osteogenesis for different time points were determined by qRT-PCR. Relative levels of osteogenesis-associated genes, including ALP, RUNX2, and Osterix, in DEX-induced MSCs overexpressing Apod or not were examined. Moreover, the protein level of RUNX2, ALP, and Osterix; ALP activity; and mineralization ability influenced by Apod in osteogenic MSCs were assessed. At last, the potential influences of Apod on the PI3K/Akt pathway were identified through detecting the expression levels of PI3K and Akt in MSCs by Western blot.
resultsApod was time-dependently upregulated in MSCs undergoing osteogenesis. DEX induction downregulated ALP, RUNX2, and Osterix and attenuated ALP activity and mineralization ability in MSCs undergoing osteogenesis, which were partially reversed by overexpression of Apod. In addition, Apod overexpression upregulated the reduced levels of PI3K and Akt in DEX-induced MSCs.
conclusionApod alleviates glucocorticoid-induced osteogenesis suppression in MSCs via the PI3K/Akt pathway, thus protecting the progression of OP.
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