Evidence map›Paper›PMID 32770507›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach.

Laureano D Asico, Selim Rozyyev, Annabelle M Crusan, Pedro A Jose, Van Anthony M Villar

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Laureano D AsicoDivision of Renal Diseases and Hypertension, School of Medicine and Health Sciences, The George Washington University, Washington, DC, USA. lasico@gwu.edu.
Selim RozyyevDivision of Renal Diseases and Hypertension, School of Medicine and Health Sciences, The George Washington University, Washington, DC, USA.
Annabelle M CrusanResearch Animal Facility, The Children's Research Institute, Children's National Health System, Washington, DC, USA.
Pedro A JoseDivision of Renal Diseases and Hypertension, School of Medicine and Health Sciences, The George Washington University, Washington, DC, USA.
Van Anthony M VillarDivision of Renal Diseases and Hypertension, School of Medicine and Health Sciences, The George Washington University, Washington, DC, USA. vvillar@gwu.edu.
George Washington University · USChildren's National · US

Funding

Spatiotemporal transregulation of D1-like angiotensin receptors in genetically...P01HL074940 · NHLBI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI GILDEA, JOHN JOSEPH · 2004 to 2020
$31.8M
RENAL DOPAMINE-1 RECEPTOR DEFECT IN HYPERTENSIONR01DK039308 · NIDDK · UNIVERSITY OF MARYLAND BALTIMORE · PI JOSE, PEDRO A. · 1987 to 2020
$7.4M
Lipid rafts, dopamine 1 receptor, and hypertensionR01DK119652 · NIDDK · GEORGE WASHINGTON UNIVERSITY · PI JOSE, PEDRO A. · 2020 to 2023
$2.6M
NHLBI NIH HHS P01 HL074940NIDDK NIH HHS R01 DK039308NIDDK NIH HHS R01 DK119652
6 · The paper itself

Abstract

Numerous G protein-coupled receptors (GPCRs) and GPCR-signaling molecules reside in lipid rafts and thus, are inherently regulated in these microdomains. However, the limitations of current methods to investigate lipid raft biology and GPCR activity in situ have hindered the complete understanding of the molecular underpinnings of GPCR trafficking and signaling, especially in the whole organism. This book chapter details an innovative in vivo approach to study the crucial role of lipid rafts on the workings of GPCRs in the mouse kidney. This protocol involves the use of a modified mini osmotic pump to deliver an agent that selectively disrupts the lipid raft in the kidney.

Indexed as

AnimalsKidneyMembrane LipidsMembrane MicrodomainsMiceProtein TransportReceptors, G-Protein-CoupledSignal TransductionMembrane LipidsReceptors, G-Protein-CoupledKidneyLipid raftMethyl-β-cyclodextrinMini osmotic pumpMouseα-Cyclodextrin

Identifiers

PMID32770507
PMCPMC7597372
OpenAlexW3047841189

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.