ArticleNature communications2020
A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Trial
- Advances in the Preparation, Characterization, and Bioactivity of Oligosaccharides From Traditional Chinese Medicine Polysaccharides: A Comprehensive Review.Chemistry & biodiversity · 2026Review
- Regulating polysaccharide structure and bioactivity via free radical degradation: a review of mechanisms, effects, and application prospects.Frontiers in nutrition · 2026Review
- Glycoside hydrolase-mediated glucomannan catabolism inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Fibre: The Forgotten Carbohydrate in Sports Nutrition Recommendations.Sports medicine (Auckland, N.Z.) · 2025Review
- Exploring the In Vivo Fate of β-1, 3/1, 6-Glucan Using Quantitative Tandem Mass Spectrometry Based on a Structure-Specific Fragment.Marine drugs · 2025Article
- Generation of novel prebiotic oligosaccharide pools from fiber drives biological insight in bacterial glycan metabolism.Applied and environmental microbiology · 2025Article
- Mass spectrometric profiling of microbial polysaccharides using laser desorption/ionization - time-of-flight (LDI-TOF) and liquid chromatography-mass spectrometry (LC-MS): a novel method for structural fingerprinting and derivatization.Frontiers in cellular and infection microbiology · 2025Article
- Polysaccharide-Based Drug Carriers-A Patent Analysis.Gels (Basel, Switzerland) · 2024Review
- A multi-glycomic platform for the analysis of food carbohydrates.Nature protocols · 2024Review
- Dietary fiber monosaccharide content alters gut microbiome composition and fermentation.Applied and environmental microbiology · 2024Article
- Food carbohydrates in the gut: structural diversity, microbial utilization, and analytical strategies.Food science and biotechnology · 2024Review
- Permethylation as a strategy for high-molecular-weight polysaccharide structure analysis by nuclear magnetic resonance-Case study of Xylella fastidiosa extracellular polysaccharide.Magnetic resonance in chemistry : MRC · 2024Article
- Structural Characterization and Anticoagulant Activities of a Keratan Sulfate-like Polysaccharide from the Sea CucumberMarine drugs · 2023Article
- Acetylation in Ionic Liquids Dramatically Increases Yield in the Glycosyl Composition and Linkage Analysis of Insoluble and Acidic Polysaccharides.Analytical chemistry · 2023Article
- Analysis of Cell Glycogen with Quantitation and Determination of Branching Using Liquid Chromatography-Mass Spectrometry.Analytical chemistry · 2023Article
- Bioactive glycans in a microbiome-directed food for malnourished children.medRxiv : the preprint server for health sciences · 2023Article
- Quantitative estimation of enzymatic released specific oligosaccharides fromJournal of pharmaceutical analysis · 2023Article
- Quantitative Bottom-Up Glycomic Analysis of Polysaccharides in Food Matrices Using Liquid Chromatography-Tandem Mass Spectrometry.Analytical chemistry · 2023Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions. Polysaccharides cannot be simply sequenced because they are often highly branched and lack a uniform structure. Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins. While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion. We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides. The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput. The technique is first used to identify polysaccharides by oligosaccharide fingerprinting. Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.