ArticleScientific reports2020
An ambient temperature collection and stabilization strategy for canine microbiota studies.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Phylogenetic influence on gut microbiome diversity within an African herbivore community.BMC ecology and evolution · 2025Article
- Best practices for the experimental design of one health studies on companion animal and owner microbiomes - From data collection to analysis.One health (Amsterdam, Netherlands) · 2025Review
- Comparison of mucosal microbiota populations across the gastrointestinal tract of healthy dogs.Animal microbiome · 2025Article
- The gut microbiota facilitate their host tolerance to extreme temperatures.BMC microbiology · 2024Article
- Effect size of delayed freezing, diurnal variation, and hindgut location on the mouse fecal microbiome.iScience · 2024Article
- Behavioral comorbidities treatment by fecal microbiota transplantation in canine epilepsy: a pilot study of a novel therapeutic approach.Frontiers in veterinary science · 2024Article
- Article
- Impact of fecal sample preservation and handling techniques on the canine fecal microbiota profile.PloS one · 2024Article
- Validation of method for faecal sampling in cats and dogs for faecal microbiome analysis.BMC veterinary research · 2023Article
- Homemade Nucleic Acid Preservation Buffer Proves Effective in Preserving the Equine Faecal Microbiota over Time at Ambient Temperatures.Animals : an open access journal from MDPI · 2023Article
- Identification of a Sub-ClinicalAnimals : an open access journal from MDPI · 2023Article
- Stabilization of swine faecal samples influences taxonomic and functional results in microbiome analyses.MethodsX · 2022Article
- On the Variability of Microbial Populations and Bacterial Metabolites within the Canine Stool. An in-Depth Analysis.Animals : an open access journal from MDPI · 2021Article
- Visualizing the Growth and Division of Rat Gut Bacteria by D-Amino Acid-BasedFrontiers in molecular biosciences · 2021Article
- Effect of esomeprazole with and without a probiotic on fecal dysbiosis, intestinal inflammation, and fecal short-chain fatty acid concentrations in healthy dogs.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Similar to humans, the fecal microbiome of dogs may be useful in diagnosing diseases or assessing dietary interventions. The accuracy and reproducibility of microbiome data depend on sample integrity, which can be affected by storage methods. Here, we evaluated the ability of a stabilization device to preserve canine fecal samples under various storage conditions simulating shipping in hot or cold climates. Microbiota data from unstabilized samples stored at room temperature (RT) and samples placed in PERFORMAbiome·GUT collection devices (PB-200) (DNA Genotek, Inc. Ottawa, Canada) and stored at RT, 37 °C, 50 °C, or undergoing repeated freeze-thaw cycles, were compared with freshly extracted samples. Alpha- and beta diversity indices were not affected in stabilized samples, regardless of storage temperature. Unstabilized samples stored at RT, however, had higher alpha diversity. Moreover, the relative abundance of dominant bacterial phyla (Firmicutes, Fusobacteria, Bacteriodetes, and Actinobacteria) and 24 genera were altered in unstabilized samples stored at RT, while microbiota abundance was not significantly changed in stabilized samples stored at RT. Our results suggest that storage method is important in microbiota studies and that the stabilization device may be useful in maintaining microbial profile integrity, especially for samples collected off-site and/or those undergoing temperature changes during shipment or storage.
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