ArticleGene therapy2021
Detection of non-targeted transgenes by whole-genome resequencing for gene-doping control.
Article in Gene therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Codon changes challenge PCR-based gene doping detection.Gene therapy · 2025Article
- Development of Detection Method Using Dried Blood Spot with Next-Generation Sequencing and LabDroid for Gene Doping Control.International journal of molecular sciences · 2025Article
- Gene doping in horse racing and equine sports: Current landscape and future perspectives.Equine veterinary journal · 2025Review
- PCR-Based Equine Gene Doping Test for the Australian Horseracing Industry.International journal of molecular sciences · 2024Article
- A Review of Recent Progress in Drug Doping and Gene Doping Control Analysis.Molecules (Basel, Switzerland) · 2023Review
- Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests.Journal of equine science · 2023Article
- Article
- Control of gene doping in human and horse sports.Gene therapy · 2022Article
- Transcriptomic Markers of Recombinant Human Erythropoietin Micro-Dosing in Thoroughbred Horses.Genes · 2021Article
- Whole-genome resequencing using genomic DNA extracted from horsehair roots for gene-doping control in horse sports.Journal of equine science · 2020Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene doping has raised concerns in human and equestrian sports and the horseracing industry. There are two possible types of gene doping in the sports and racing industry: (1) administration of a gene-doping substance to postnatal animals and (2) generation of genetically engineered animals by modifying eggs. In this study, we aimed to identify genetically engineered animals by whole-genome resequencing (WGR) for gene-doping control. Transgenic cell lines, in which the erythropoietin gene (EPO) cDNA form was inserted into the genome of horse fibroblasts, were constructed as a model of genetically modified horse. Genome-wide screening of non-targeted transgenes was performed to find structural variation using DELLY based on split-read and paired-end algorithms and Control-FREEC based on read-depth algorithm. We detected the EPO transgene as an intron deletion in the WGR data by the split-read algorithm of DELLY. In addition, single-nucleotide polymorphisms and insertions/deletions artificially introduced in the EPO transgene were identified by WGR. Therefore, genome-wide screening using WGR can contribute to gene-doping control even if the targets are unknown. This is the first study to detect transgenes as intron deletions for gene-doping detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.