ArticleScientific reports2020
Fully biological production of adipic acid analogs from branched catechols.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Microbial Lignin Valorization to Protocatechuic Acid and Catechol: Biofunneling Pathways and Metabolic Engineering Strategies.Biomolecules · 2026Review
- Unlocking microbial potential: advances in omics and bioinformatics for aromatic hydrocarbon degradation.World journal of microbiology & biotechnology · 2025Review
- Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-freeEngineering microbiology · 2025Article
- Recent advances of muconic acid production using microbial synthetic biology.Archives of microbiology · 2025Review
- Microbial Production of Fuels, Commodity Chemicals, and Materials from Sustainable Sources of Carbon and Energy.Current opinion in systems biology · 2023Article
- Physical Pretreatments Applied in Three Commercial Kits for the Extraction of High-Quality DNA from Activated Sewage Sludge.International journal of molecular sciences · 2023Article
- Divergent directed evolution of a TetR-type repressor towards aromatic molecules.Nucleic acids research · 2023Article
- Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid.Frontiers in microbiology · 2023Article
- Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives.Journal of fungi (Basel, Switzerland) · 2021Review
- Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-Chem catalysis · 2021Article
- Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid.Microbial cell factories · 2021Article
- Environmental benefits of soy-based bio-adhesives as an alternative to formaldehyde-based options.Environmental science and pollution research international · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial production of adipic acid from lignin-derived monomers, such as catechol, is a greener alternative to the petrochemical-based process. Here, we produced adipic acid from catechol using catechol 1,2-dioxygenase (CatA) and a muconic acid reductase (MAR) in Escherichia coli. As the reaction progressed, the pH of the media dropped from 7 to 4-5 and the muconic acid isomerized from the cis,cis (ccMA) to the cis,trans (ctMA) isomer. Feeding experiments suggested that cells preferentially uptook ctMA and that MAR efficiently reduced all muconic isomers to adipic acid. Intrigued by the substrate promiscuity of MAR, we probed its utility to produce branched chiral diacids. Using branched catechols likely found in pretreated lignin, we found that while MAR fully reduced 2-methyl-muconic acid to 2-methyl-adipic acid, MAR reduced only one double bond in 3-substituted muconic acids. In the future, MAR's substrate promiscuity could be leveraged to produce chiral-branched adipic acid analogs to generate branched, nylon-like polymers with reduced crystallinity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.