Evidence map›Paper›PMID 32768236›Full record

ArticleVeterinary microbiology2020

Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication.

Pengfei Zhang, Linyang Yu, Jianguo Dong, Yanling Liu, Leyi Zhang, Pengshuai Liang, Lei Wang, Bin Chen, Li Huang, Changxu Song

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Pengfei ZhangCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China.
Linyang YuCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China.
Jianguo DongSchool of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, China.
Yanling LiuCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China.
Leyi ZhangCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China.
Pengshuai LiangCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China.
Lei WangCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China.
Bin ChenSchool of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, China.
Li HuangSchool of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, China. Electronic address: cnvet19@126.com.
Changxu SongCollege of Animal Science & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou, 510642, China. Electronic address: cxsong2004@163.com.
South China Agricultural University · CNXinyang College of Agriculture and Forestry · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) belongs to the Alphacoronavirus genus in the Coronaviridae family. Similar to other coronaviruses, PEDV encodes two papain-like proteases. Papain-like protease (PLP)2 has been proposed to play a key role in antagonizing host innate immunity. However, the function of PLP1 remains unclear. In this study, we found that overexpression of PLP1 significantly promoted PEDV replication and inhibited production of interferon-β. Immunoprecipitation and mass spectrometry were used to identify cellular interaction partners of PLP1. Host cell poly(C) binding protein 2 (PCBP2) was determined to bind and interact with PLP1. Both endogenous and overexpressed PCBP2 co-localized with PLP1 in the cytoplasm. Overexpression of PLP1 upregulated expression of PCBP2. Furthermore, overexpression of PCBP2 promoted PEDV replication. Silencing of endogenous PCBP2 using small interfering RNAs attenuated PEDV replication. Taken together, these data demonstrated that PLP1 negatively regulated the production of type 1 interferon by interacting with PCBP2 and promoted PEDV replication.

Indexed as

AnimalsChlorocebus aethiopsCoronavirus Papain-Like ProteasesGene Expression RegulationHEK293 CellsHumansInterferon-betaMyelin Proteolipid ProteinPapainPorcine epidemic diarrhea virusRNA-Binding ProteinsRNA InterferenceTumor Necrosis Factor-alphaVero CellsViral Nonstructural ProteinsVirus ReplicationCoronavirus Papain-Like ProteasesInterferon-betaMyelin Proteolipid ProteinPapainPLP1 protein, humanRNA-Binding ProteinsTumor Necrosis Factor-alphaViral Nonstructural ProteinsCoronavirusInteractionPapain-like proteasesPEDVPoly(C) binding protein 2Virus replication

Identifiers

PMID32768236
PMCPMC7355335
OpenAlexW3043331492

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.