ArticleMicrobial biotechnology2020
First description of betalains biosynthesis in an aquatic organism: characterization of 4,5-DOPA-extradiol-dioxygenase activity in the cyanobacteria Anabaena cylindrica.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 31 citations in OpenAlex.
- Advances in Betalain Biosynthesis and Metabolic Engineering for Sustainable Natural Pigment Production.Biotech (Basel (Switzerland)) · 2026Review
- A functional betalain-producing dioxygenase inIMA fungus · 2026Article
- Transcription Factors AsMYB1 and AsMYB3 Regulate Betalain Biosynthesis inJournal of fungi (Basel, Switzerland) · 2025Article
- In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase.PloS one · 2025Article
- High-efficiency production of plant-derived pigment dopaxanthin in Escherichia coli by combination engineering.Microbial cell factories · 2024Article
- Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot.BMC plant biology · 2023Article
- Combinatorial engineering of betalain biosynthesis pathway in yeast Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Large-scale phylogenomics of aquatic bacteria reveal molecular mechanisms for adaptation to salinity.Science advances · 2023Article
- Reannotation of Fly Amanita l-DOPA Dioxygenase Gene Enables Its Cloning and Heterologous Expression.ACS omega · 2022Article
- First description of betalains biosynthesis in an aquatic organism: characterization of 4,5-DOPA-extradiol-dioxygenase activity in the cyanobacteria Anabaena cylindrica.Microbial biotechnology · 2020Article
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3 authors.
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Abstract
The biosynthesis of betalamic acid, the structural unit of pigments betalains, is performed by enzymes with 4,5-DOPA-extradiol-dioxygenase activity. These enzymes were believed to be limited to plants of the order Caryophyllales and to some fungi. However, the discovery of Gluconacetobacter diazotrophicus as the first betalain-forming bacterium opened a new field in the search for novel biological systems able to produce betalains. This paper describes molecular and functional characterization of a novel dioxygenase enzyme from the aquatic cyanobacterium Anabaena cylindrica. The enzyme was found to be a homodimer of a polypeptide of 17.8 kDa that, opposite to previous related enzymes, showed a strong inhibition by excess of the precursor L-DOPA. However, its heterologous expression has allowed detecting the formation of the main compounds in the biosynthetic pathway of betalains. In addition, phylogenetic analysis has shown that this enzyme is not close related to enzymes from plants, fungi or proteobacteria such as G. diazotrophicus. The presence of enzymes that produce these health-promoting compounds is more diverse than expected. The discovery of this novel dioxygenase in the phylum cyanobacteria expands the presence of betalamic acid-forming enzymes in organisms of different nature with no apparent relationship among them.
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