Evidence map›Paper›PMID 32767207›Full record

ArticleJournal of assisted reproduction and genetics2020

Discordance between human sperm quality and telomere length following differential gradient separation/swim-up.

Ana Catarina Lopes, Pedro Fontes Oliveira, Soraia Pinto, Carolina Almeida, Maria João Pinho, Rosália Sá, Eduardo Rocha, Alberto Barros, Mário Sousa

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 2 pooled it
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 2 syntheses or guidelines pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Review
  6. Review
  7. Telomere Length in Metaphase Chromosomes of Human Triploid Zygotes.International journal of molecular sciences · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Ana Catarina LopesLaboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.ORCID https://orcid.org/0000-0003-0104-8199
Pedro Fontes OliveiraLaboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.ORCID https://orcid.org/0000-0002-4989-5699
Soraia PintoCentre for Reproductive Genetics Prof. Alberto Barros, 4100-009, Porto, Portugal.ORCID https://orcid.org/0000-0002-8810-1978
Carolina AlmeidaDepartment of Genetics, Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal.ORCID https://orcid.org/0000-0002-9809-0651
Maria João PinhoDepartment of Genetics, Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal.ORCID https://orcid.org/0000-0003-3142-4351
Rosália SáLaboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.ORCID https://orcid.org/0000-0002-6551-3822
Eduardo RochaLaboratory of Histology and Embryology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar, University of Porto, 4050-313, Porto, Portugal.ORCID https://orcid.org/0000-0001-9813-4389
Alberto BarrosDepartment of Genetics, Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal.ORCID https://orcid.org/0000-0002-8700-3698
Mário SousaLaboratory of Cell Biology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. msousa@icbas.up.pt.ORCID https://orcid.org/0000-0002-3009-3290
Universidade do Porto · PTCentro de Genética Clínica · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStrong evidence has suggested an important role of telomeres in meiosis, fertilization, and embryo development. PURPOSE: To determine if sperm telomere length (STL) in sperm purified by differential gradient centrifugation followed by swim-up (selected STL) is correlated with sperm quality and clinical outcomes.

methodsRelative selected STL was assessed by quantitative polymerase chain reaction (Q-PCR) in 78 consecutive assisted reproductive technology (ART) treatments during 2017. Statistical analyses were performed in the totality of patients, and in normozoospermic and non-normozoospermic patients. These included correlations between selected STL and sperm quality parameters, embryological parameters (multivariable linear regression), and clinical parameters (multivariable logistic regression).

resultsNo significant correlations were found between selected STL and sperm quality in the total population. However, selected STL was significantly correlated with total sperm count (r = 0.361; P = 0.039) and sperm DNA fragmentation-post-acrosomal region pattern (r = - 0.464; P = 0.030) in normozoospermic patients. No relation was observed between selected STL and clinical outcomes in any clinical group.

conclusionsAs the correlations observed in normozoospermic patients were not representative of the whole heterogeneous population, differences in the sperm characteristics of the study population may lead to discrepant results when evaluating the association of STL with sperm quality. Since the total population selected STL was not related with sperm quality and with clinical outcomes, results do not support the use of selected STL measurement to evaluate the reproductive potential of the male patient or to predict the success rates of ART treatments.

Indexed as

Reproductive Techniques, AssistedAdultCentrifugation, Density GradientHumansMaleMiddle AgedSpermatozoaSperm CountSperm MotilityTelomereTelomere HomeostasisAssisted reproductionInfertility biomarkersMale infertilitySperm telomere length

Identifiers

PMID32767207
PMCPMC7550483
OpenAlexW3048164480

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.