ArticleJournal of assisted reproduction and genetics2020
Embryonic MTHFR contributes to blastocyst development.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- One-carbon metabolism and risk of PCOS: insights from Mendelian randomization.Journal of ovarian research · 2025Article
- Key biochemical pathways during pregnancy in livestock: mechanisms regulating uterine and placental development and function.Reproduction & fertility · 2025Review
- SHMT2 is essential for mammalian preimplantation embryonic development throughMolecular therapy. Nucleic acids · 2025Article
- MTHFR as a Novel Candidate Marker for Litter Size in Rabbits.Animals : an open access journal from MDPI · 2024Article
- Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak.Frontiers in veterinary science · 2024Article
- Genome-wide profiling of histone H3K4me3 and H3K27me3 modifications in individual blastocysts by CUT&Tag without a solid support (NON-TiE-UP CUT&Tag).Scientific reports · 2022Article
- Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- MTHFR (methylenetetrahydrofolate reductase: EC 1.5.1.20) SNPs (single-nucleotide polymorphisms) and homocysteine in patients referred for investigation of fertility.Journal of assisted reproduction and genetics · 2021Article
- Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle.Scientific reports · 2021Article
- Methylation: An Ineluctable Biochemical and Physiological Process Essential to the Transmission of Life.International journal of molecular sciences · 2020Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeReduction in methylenetetrahydrofolate reductase (MTHFR) activity due to genetic variations in the MTHFR gene has been controversially implicated in subfertility in human in vitro fertilization. However, there is no direct gene-knockdown study of embryonic MTHFR to assess its involvement in mammalian preimplantation development. The purpose of this study is to investigate expression profiles and functional roles of MTHFR in bovine preimplantation development.
methodsReverse transcription-quantitative PCR (RT-qPCR) and analysis of publicly available RNA-seq data were performed to reveal expression levels of MTHFR during bovine preimplantation development. We knocked down MTHFR by siRNA-mediated RNA interference from the 8- to 16-cell stage and assessed the effects on preimplantation development.
resultsThe RT-qPCR analysis showed relatively high MTHFR expression at the GV oocyte stage, which was decreased toward the 8- to 16-cell stage and then slightly restored at the blastocyst stage. Public data-based analysis also showed the similar pattern of expression with substantial embryonic expression at the blastocyst stage. MTHFR knockdown reduced the blastocyst rate (P < 0.01) and the numbers of total (P < 0.0001), trophectoderm (P < 0.0001), and inner cell mass (P < 0.001) cells.
conclusionThe results indicate that embryonic MTHFR is indispensable for normal blastocyst development. The findings provide insight into the debatable roles of MTHFR in fertility and may be applicable for the improvement of care for early embryos via modulation of surrounding folate-related nutritional conditions in vitro and/or in utero, depending on the parental and embryonic MTHFR genotype.
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