ArticleThe Journal of cell biology1988
Microfilament modification by dihydrocytochalasin B causes retinoic acid-modulated chondrocytes to reexpress the differentiated collagen phenotype without a change in shape.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 187 citations in OpenAlex.
- Targeting F-actin stress fibers to suppress the dedifferentiated phenotype in chondrocytes.European journal of cell biology · 2024Article
- Hypertrophic chondrocytes at the junction of musculoskeletal structures.Bone reports · 2023Article
- Superficial and deep zone articular chondrocytes exhibit differences in actin polymerization status and actin-associated moleculesOsteoarthritis and cartilage open · 2020Article
- Patient-reported health outcomes for severe knee osteoarthritis after conservative treatment with an intra-articular cell-free formulation for articular cartilage regeneration combined with usual medical care vs. usual medical care alone: A randomized controlled trial.Experimental and therapeutic medicine · 2019Article
- Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation.Molecular medicine reports · 2018Article
- Interplay between cytoskeletal polymerization and the chondrogenic phenotype in chondrocytes passaged in monolayer culture.Journal of anatomy · 2017Article
- Berberine induces dedifferentiation by actin cytoskeleton reorganization via phosphoinositide 3-kinase/Akt and p38 kinase pathways in rabbit articular chondrocytes.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- Shape of chondrocytes within articular cartilage affects the solid but not the fluid microenvironment under unconfined compression.Acta biomaterialia · 2016Article
- Interleukin-1β and tumor necrosis factor-α increase stiffness and impair contractile function of articular chondrocytes.Acta biochimica et biophysica Sinica · 2015Article
- Culture temperature affects human chondrocyte messenger RNA expression in monolayer and pellet culture systems.PloS one · 2015Article
- Surface zone articular chondrocytes modulate the bulk and surface mechanical properties of the tissue-engineered cartilage.Tissue engineering. Part A · 2014Article
- Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton.Tissue engineering. Part A · 2014Article
- The morphology and functions of articular chondrocytes on a honeycomb-patterned surface.BioMed research international · 2014Article
- Maximizing phenotype constraint and extracellular matrix production in primary human chondrocytes using arginine-glycine-aspartate concentration gradient hydrogels.Acta biomaterialia · 2013Article
- Primary human chondrocyte extracellular matrix formation and phenotype maintenance using RGD-derivatized PEGDM hydrogels possessing a continuous Young's modulus gradient.Acta biomaterialia · 2013Article
- Brief daily exposure to low-intensity vibration mitigates the degradation of the intervertebral disc in a frequency-specific manner.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Article
- The effect of Lidocaine on the viability of cultivated mature human cartilage cells: an in vitro study.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2011Article
- Control of chondrocyte gene expression by actin dynamics: a novel role of cholesterol/Ror-alpha signalling in endochondral bone growth.Journal of cellular and molecular medicine · 2009Article
- Rho kinase-dependent CCL20 induced by dynamic compression of human chondrocytes.Arthritis and rheumatism · 2008Article
- Mechanical control of tissue morphogenesis.Circulation research · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Primary monolayers of rabbit articular chondrocytes synthesize high levels of type II collagen and proteoglycan. This capacity was used as a marker for the expression of the differentiated phenotype. Such cells were treated with 1 microgram/ml retinoic acid (RA) for 10 d to produce a modulated collagen phenotype devoid of type II and consisting of predominantly type I trimer and type III collagen. After transfer to secondary culture in the presence of RA, the stability of the RA-modulated phenotype was investigated by culture in the absence of RA. Little reexpression of type II collagen synthesis occurred in this period unless cultures were treated with 3 X 10(-6) M dihydrocytochalasin B to modify microfilament structures. Reexpression of the differentiated phenotype began between days 6-8 and was essentially complete by day 14. Substantial reexpression occurred by day 8 without a detectable increase in cell rounding. Colony formation, characteristic of primary chondrocytes, was infrequent even after reexpression was complete. These data suggest that the integrity of microfilament cytoskeletal structures can be a source of regulatory signals that mechanistically appear to be more proximal to phenotypic change than the overt changes in cell shape that accompany reexpression of subculture-modulated chondrocytes in agarose culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.