Evidence map›Paper›PMID 32766980›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Mast Cell Activation Test (MAT).

Rajia Bahri, Silvia Bulfone-Paus

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Cells · 2026
    Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Role of Mast Cells in Eosinophilic Gastrointestinal Diseases.Immunology and allergy clinics of North America · 2024
    Review
  8. Article
  9. Hypersensitivity may be involved in severe COVID-19.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2022
    Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Rajia BahriLydia Becker Institute of Immunology and Inflammation, Division of Musculoskeletal and Dermatological Sciences & Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
Silvia Bulfone-PausLydia Becker Institute of Immunology and Inflammation, Division of Musculoskeletal and Dermatological Sciences & Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. silvia.bulfone-paus@manchester.ac.uk.

Funding

Medical Research Council MC-PC-15038
6 · The paper itself

Abstract

The mast cell (MC) activation assay is a robust in vitro tool for exploring MC reactivity in allergy. Here we describe the use of the mast cell activation test (MAT) that makes use of human primary MCs generated from peripheral blood progenitors, sensitized overnight with patients' sera and activated with allergens. Flow cytometry is used to assess the changes in expression of the activation marker CD63, and the percentage of cell degranulation is defined as the percentage of CD63

Indexed as

AllergensAnimalsCell Culture TechniquesCell DegranulationCells, CulturedFlow CytometryHumansHypersensitivityImmunoglobulin ELeukocytes, MononuclearMast CellsTetraspanin 30AllergensCD63 protein, humanImmunoglobulin ETetraspanin 30Allergic diseaseAllergic patient seraFlow cytometryMast cellMast cell activationPeripheral blood progenitor

Identifiers

PMID32766980

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.