ArticleJournal of radiation research2020
Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining.
Article in Journal of radiation research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Development of a DNA marker for cultivar identification of "Harushizuka", a new satsuma mandarin cultivar created by heavy-ion irradiation.Breeding science · 2026Article
- Manipulating alternative end-joining alters carbon-ion beam-induced genome mutation profiles in Arabidopsis thaliana.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
- Exploring the complexity of genome size reduction in angiosperms.Plant molecular biology · 2024Review
- Genomic view of heavy-ion-induced deletions associated with distribution of essential genes inFrontiers in plant science · 2024Article
- Production of Enhanced Carotenoid-Producing Strains of the Yeast Rhodotorula gracilis Using the Antibiotic Zeocin.Applied biochemistry and biotechnology · 2023Article
- Physiological and Transcriptomic Analyses Reveal the Effects of Carbon-Ion Beam onInternational journal of molecular sciences · 2023Article
- Comparative analysis of seed and seedling irradiation with gamma rays and carbon ions for mutation induction in Arabidopsis.Frontiers in plant science · 2023Article
- Distinct mechanisms for genomic attachment of the 5' and 3' ends of Agrobacterium T-DNA in plants.Nature plants · 2022Article
- Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing inInternational journal of molecular sciences · 2022Article
- Detection and characterization of genome-wide mutations in M1 vegetative cells of gamma-irradiated Arabidopsis.PLoS genetics · 2022Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the involvement of the non-homologous end joining (NHEJ) pathway in plant mutagenesis by ionizing radiation, we conducted a genome-wide characterization of the mutations induced by gamma rays in NHEJ-deficient Arabidopsis mutants (AtKu70-/- and AtLig4-/-). Although both mutants were more sensitive to gamma rays than the wild-type control, the AtKu70-/- mutant was slightly more sensitive than the AtLig4-/- mutant. Single-base substitutions (SBSs) were the predominant mutations in the wild-type control, whereas deletions (≥2 bp) and complex-type mutations [i.e. more than two SBSs or short insertion and deletions (InDels) separated by fewer than 10 bp] were frequently induced in the mutants. Single-base deletions were the most frequent deletions in the wild-type control, whereas the most common deletions in the mutants were 11-30 bp. The apparent microhomology at the rejoined sites of deletions peaked at 2 bp in the wild-type control, but was 3-4 bp in the mutants. This suggests the involvement of alternative end joining and single-strand annealing pathways involving increased microhomology for rejoining DNA ends. Complex-type mutations comprising short InDels were frequently detected in the mutants, but not in the wild-type control. Accordingly, NHEJ is more precise than the backup pathways, and is the main pathway for rejoining the broken DNA ends induced by ionizing radiation in plants.
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