Evidence map›Paper›PMID 32766708›Full record

ArticleBioscience reports2020

Circ_0000144 functions as a miR-623 sponge to enhance gastric cancer progression via up-regulating GPRC5A.

Lili Mi, Lianhui Lei, Xiaolei Yin, Ning Li, Jianfei Shi, Xin Han, Xiaoling Duan, Man Zhao, Guangjie Han, Jinfeng Wang and 1 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Non-coding RNA in cancer.Essays in biochemistry · 2021
    Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Lili MiDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Lianhui LeiDepartment of Surgery, China Electronics Technology Group Corporation No.54 Staff Hospital, Shijiazhuang, Hebei 050011, China.
Xiaolei YinDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Ning LiDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Jianfei ShiDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Xin HanDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Xiaoling DuanDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Man ZhaoDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Guangjie HanDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Jinfeng WangDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Fei YinDepartment of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Hebei Medical University · CNChina Electronics Technology Group Corporation · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGastric cancer (GC) remains one of the most common malignancies worldwide. Increasing evidence has demonstrated that circRNAs serve as critical roles in human cancer, including GC. In the present study, we focused on the detailed function and mechanism of circ_0000144 on GC progression.

methodsThe levels of circ_0000144, miR-623 and G-protein-coupled receptor, family C, group 5, member A (GPRC5A) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Targeted relationships among circ_0000144, miR-623 and GPRC5A were confirmed using dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Cell proliferation, colony formation, apoptosis, migration and invasion were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, flow cytometry and transwell assays. Measurement of glutamine and α-ketoglutarate (α-KG) levels was performed using a corresponding assay kit. GPRC5A protein expression was detected using Western blot. In vivo assays were used to explore the impact of circ_0000144 on tumor growth.

resultsOur data indicated that circ_0000144 was up-regulated and miR-623 was down-regulated in GC tissues and cells. Circ_0000144 interacted with miR-623 through directly binding to miR-623. Moreover, the knockdown of circ_0000144 weakened GC cell proliferation, colony formation, migration, invasion and glutaminolysis and accelerated cell apoptosis by up-regulating miR-623. GPRC5A was a direct target of miR-623 and circ_0000144 protected against GPRC5A repression through sponging miR-623. Furthermore, miR-623-mediated regulation on GC cell progression was reversed by the stored expression of GPRC5A. Additionally, circ_0000144 depletion inhibited tumor growth in vivo.

conclusionOur study indicated that circ-0000144 knockdown repressed GC progression at least partly by regulating GPRC5A expression via sponging miR-623, illumining a novel therapeutic target for GC treatment.

Indexed as

AnimalsApoptosisCell Line, TumorCell MovementCell ProliferationDisease ProgressionGene Expression Regulation, NeoplasticHumansMaleMice, Inbred BALB CMice, NudeMicroRNAsNeoplasm InvasivenessReceptors, G-Protein-CoupledRNA, CircularSignal TransductionGPRC5A protein, humanMicroRNAsMIRN623 microRNA, humanReceptors, G-Protein-CoupledRNA, Circularcirc_0000144GCGPRC5AmiR-623

Identifiers

PMID32766708
PMCPMC7426631
OpenAlexW3048284246

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.