Evidence map›Paper›PMID 32766604›Full record

ArticlemedRxiv : the preprint server for health sciences2020

A blueprint for academic labs to produce SARS-CoV-2 RT-qPCR test kits.

Samantha J Mascuch, Sara Fakhretaha-Aval, Jessica C Bowman, Minh Thu H Ma, Gwendell Thomas, Bettina Bommarius, Chieri Ito, Liangjun Zhao, Gary P Newnam, Kavita R Matange and 25 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Considerations for diagnostic COVID-19 tests.Nature reviews. Microbiology · 2021
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

35 authors.

Samantha J MascuchSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
Sara Fakhretaha-AvalSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Jessica C BowmanSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Minh Thu H MaSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Gwendell ThomasSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Bettina BommariusPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, USA.
Chieri ItoSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Liangjun ZhaoSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Gary P NewnamSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Kavita R MatangeSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Hem R ThapaSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Brett BarlowSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Rebecca K DoneganSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Nguyet A NguyenSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Emily G SaccuzzoSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Chiamaka T ObianyorSchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Suneesh C KarunakaranSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Pamela PolletSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Brooke Rothschild-MancinelliSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Santi Mestre-FosSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Rebecca Guth-MetzlerSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Anton V BryksinPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, USA.
Anton S PetrovSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Mallory HazellSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Carolyn B IbbersonSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
Petar I PenevSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
Robert G ManninoWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Wilbur A LamWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Andrés J GarciaPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, USA.
Julia M KubanekSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Vinayak AgarwalSchool of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
Nicholas V HudSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Jennifer B GlassPetit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, USA.
Loren Dean WilliamsSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Raquel L LiebermanSchool of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.

Funding

Technology Training and Dissemination CoreU54EB027690 · NIBIB · EMORY UNIVERSITY · PI Wilbur A Lam, GREGORY S MARTIN · 2018 to 2026
$96.1M
NIBIB NIH HHS U54 EB027690
6 · The paper itself

Abstract

Widespread testing for the presence of the novel coronavirus SARS-CoV-2 in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise and/or instrumentation necessary to detect the virus by quantitative reverse transcription polymerase chain reaction (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably to a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces across various campus laboratories for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings.

Indexed as

DNA polymeraseformulationpolymerase chain reactionreverse transcriptaseribonuclease inhibitorSARS-CoV-2virus

Identifiers

PMID32766604
PMCPMC7402063

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.