ArticleFrontiers in microbiology2020
Development and Application of an MRT-qPCR Assay for Detecting Coinfection of Six Vertically Transmitted or Immunosuppressive Avian Viruses.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Pooled it
- Review
- Development and validation of a quantitative pcr array assay for the detection of viral sequences in breast cancer.Journal, genetic engineering & biotechnology · 2026Article
- Fowl adenovirus infections: A comprehensive review of prevalence, pathogenesis, diagnosis, control, and economic impact.Poultry science · 2026Review
- Article
- Development of a quadruplex RT-qPCR for the detection of avian leukosis virus, chicken infectious anemia virus, avian reovirus, and fowl adenovirus.Frontiers in veterinary science · 2026Article
- Genomic and pathogenic characterization of a highly pathogenic chicken infectious anemia virus strain in China.Frontiers in veterinary science · 2026Article
- Chicken Infectious Anemia Virus Markedly Enhances the Pathogenicity of Infectious Bronchitis Virus-Infected Chickens.Transboundary and emerging diseases · 2026Article
- Current Knowledge on the Diagnostic Methods, Epidemiological Characteristics and Antiviral Strategies of Chicken Anemia Virus.Veterinary sciences · 2025Review
- IBDV-SSA, a novel molecular approach for the recovery of infectious bursal disease virus whole genomes from FTA cards.Microbiology spectrum · 2025Article
- Advances in Chicken Infectious Anemia Vaccines.Vaccines · 2025Review
- Simultaneous detection and partial molecular characterization of five RNA viruses associated with enteric disease in chickens: chicken astrovirus, avian nephritis virus, infectious bronchitis virus, avian rotavirus a and avian orthoreovirus, via multiplex RT-qPCR.Frontiers in veterinary science · 2025Article
- From surveillance to pathogenesis: characterization of genotype V of chicken infectious anemia virus.Frontiers in veterinary science · 2025Article
- A snapshot on molecular technologies for diagnosing FAdV infections.Frontiers in veterinary science · 2025Review
- Construction of Recombinant Marek's Disease Virus Co-Expressing VP1 and VP2 of Chicken Infectious Anemia Virus.Vaccines · 2024Article
- Synergy of Subgroup J Avian Leukosis Virus and Chicken Infectious Anemia Virus Enhances the Pathogenicity in Chickens.Microorganisms · 2024Article
- Isolation and genomic characterization of chicken infectious anemia virus in Jiangsu province of China during 2020-2022.Frontiers in veterinary science · 2024Article
- Gyrovirus: current status and challenge.Frontiers in microbiology · 2024Review
- Characterization and pathogenicity of a novel avian orthoreovirus in China.Frontiers in microbiology · 2024Article
- Infectious bursal disease virus: predicting viral pathotype using machine learning models focused on early changes in total blood cell counts.Veterinary research · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marek's disease virus (MDV), reticuloendotheliosis virus (REV), avian reovirus (ARV), chicken infectious anemia virus (CIAV), infectious bursal disease virus (IBDV), and fowl adenovirus (FAdV) are important causes of disease in poultry. To investigate the infection status of the above six viruses in chickens in China, 1,187 samples from chicken flocks were collected and tested using a newly developed multiplex reverse-transcription quantitative real-time PCR (MRT-qPCR) assay in the study. A series of validation tests confirmed that the MRT-qPCR assay has high specificity, sensitivity, and repeatability. As for six detected pathogens, CIAV had the highest detection ratio, while ARV was not detected in any samples. In the spleen samples, the coinfection rate for MDV and CIAV was 1.6%, and that for REV and CIAV was 0.4%. In the bursa samples, the coinfection rate for FAdV and CIAV was 0.3%, and that for IBDV and CIAV was 1%. In the thymus samples, the coinfection rates for MDV and CIAV and for REV and CIAV were both 0.8%. Our study indicates that the coinfection of these viruses was existing in chickens in China. Through the detection of clinical samples, this study provides data on the coinfections of the above six pathogens and provides a basis for the further study of viral coinfection in chickens.
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