ArticlePloS one2020
All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 34 citations in OpenAlex.
- Whole-Mount Immunostaining of Tyrosine Hydroxylase for Dopaminergic Neuron Analysis in Zebrafish Larvae.Bio-protocol · 2026Article
- Amelioration of 6-OHDA-Induced Parkinson's Symptoms in Zebrafish Larvae by an Almond Skin Acetonic Extract.International journal of molecular sciences · 2026Article
- Genomic Organization, Evolutionary Conservation and Expression ofBiomedicines · 2025Article
- Disruption ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Comprehensive 3D Imaging of Whole Zebrafish Using a Water-Based Clearing Reagent for Hard Tissues.Zebrafish · 2025Article
- The zebrafish in toxicology: a bibliometric analysis reveals current trends and future avenues for predictive safety assessment.Frontiers in toxicology · 2025Review
- Conserved glucokinase regulation in zebrafish confirms therapeutic utility for pharmacologic modulation in diabetes.Communications biology · 2024Article
- ComparativeBioTechniques · 2022Article
- Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development.BMC genomics · 2022Article
- Functional identification of the zebrafish Interleukin-1 receptor in an embryonic model of Il-1β-induced systemic inflammation.Frontiers in immunology · 2022Article
- Structural Analysis and Spatiotemporal Expression ofInternational journal of molecular sciences · 2021Article
- In Silico Target Prediction of Overexpressed microRNAs from LPS-Challenged Zebrafish (International journal of molecular sciences · 2021Article
- Behavioral and Gene Regulatory Responses to Developmental Drug Exposures in Zebrafish.Frontiers in psychiatry · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zebrafish Danio rerio is a valuable and common model for scientists in the fields of genetics and developmental biology. Since zebrafish are also amenable to genetic manipulation, modelling of human diseases or behavioral experiments have moved into the focus of zebrafish research. Consequently, gene expression data beyond embryonic and larval stages become more important, yet there is a dramatic knowledge gap of gene expression beyond day four of development. Like in other model organisms, the visualization of spatial and temporal gene expression by whole mount in situ hybridization (ISH) becomes increasingly difficult when zebrafish embryos develop further and hence the growing tissues become dense and less permeable. Here we introduce a modified method for whole mount ISH, which overcomes these penetration and detection problem. The method is an all in one solution that enables the detection and visualization of gene expression patterns up to the late larval stage in a 3D manner without the need for tissue sectioning and offers a valuable extension for whole mount ISH by immunohistochemistry in the zebrafish field.
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