Evidence map›Paper›PMID 32764132›Full record

ArticlePlant physiology2020

Robust Survival-Based RNA Interference of Gene Families Using in Tandem Silencing of Adenine Phosphoribosyltransferase.

Robert G Orr, Stephen J Foley, Catherine Sherman, Isidro Abreu, Giulia Galotto, Boyuan Liu, Manuel González-Guerrero, Luis Vidali

Open access · hybridAbstract readEvaluation Study
In one paragraph

Article in Plant physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Robert G OrrDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609.
Stephen J FoleyDepartment of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, Massachusetts 01609.ORCID 0000-0002-6281-4822
Catherine ShermanDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609.
Isidro AbreuCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid 28223, Spain.ORCID 0000-0002-1647-9883
Giulia GalottoDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609.
Boyuan LiuDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609.ORCID 0000-0002-1014-9509
Manuel González-GuerreroCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid 28223, Spain.ORCID 0000-0001-7334-5286
Luis VidaliDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609 lvidali@wpi.edu.ORCID 0000-0002-5835-4587
Worcester Polytechnic Institute · USCentre for Plant Biotechnology and Genomics · ES

Funding

Composition, in vivo dynamics, and regulation of the exocyst in plant cellsR15GM134493 · NIGMS · WORCESTER POLYTECHNIC INSTITUTE · PI VIDALI, LUIS · 2019 to 2019
$448k
NIGMS NIH HHS R15 GM134493
6 · The paper itself

Abstract

RNA interference (RNAi) enables flexible and dynamic interrogation of entire gene families or essential genes without the need for exogenous proteins, unlike CRISPR-Cas technology. Unfortunately, isolation of plants undergoing potent gene silencing requires laborious design, visual screening, and physical separation for downstream characterization. Here, we developed an adenine phosphoribosyltransferase (APT)-based RNAi technology (APTi) in

Indexed as

Genetic TechniquesMultigene FamilyRNA InterferenceAdenine PhosphoribosyltransferaseBryopsidaGenes, PlantAdenine Phosphoribosyltransferase

Identifiers

PMID32764132
PMCPMC7536682
OpenAlexW3048260003

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.