Evidence map›Paper›PMID 32763163›Full record

ArticleStem cell reports2020

Direct Comparison of Four Hematopoietic Differentiation Methods from Human Induced Pluripotent Stem Cells.

Melinda L Tursky, To Ha Loi, Crisbel M Artuz, Suad Alateeq, Ernst J Wolvetang, Helen Tao, David D Ma, Timothy J Molloy

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

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  20. STAR protocols · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Melinda L TurskyBlood, Stem Cell, and Cancer Research Programme, St Vincent's Centre for Applied Medical Research and Department of Hematology and BM Transplant, St Vincent's Hospital, Level 8, 405 Liverpool Street, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia.
To Ha LoiBlood, Stem Cell, and Cancer Research Programme, St Vincent's Centre for Applied Medical Research and Department of Hematology and BM Transplant, St Vincent's Hospital, Level 8, 405 Liverpool Street, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia.
Crisbel M ArtuzBlood, Stem Cell, and Cancer Research Programme, St Vincent's Centre for Applied Medical Research and Department of Hematology and BM Transplant, St Vincent's Hospital, Level 8, 405 Liverpool Street, Darlinghurst, NSW 2010, Australia.
Suad AlateeqStem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, Brisbane, QLD 4072, Australia.
Ernst J WolvetangStem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, Brisbane, QLD 4072, Australia.
Helen TaoBlood, Stem Cell, and Cancer Research Programme, St Vincent's Centre for Applied Medical Research and Department of Hematology and BM Transplant, St Vincent's Hospital, Level 8, 405 Liverpool Street, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia.
David D MaBlood, Stem Cell, and Cancer Research Programme, St Vincent's Centre for Applied Medical Research and Department of Hematology and BM Transplant, St Vincent's Hospital, Level 8, 405 Liverpool Street, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia. Electronic address: d.ma@amr.org.au.
Timothy J MolloyBlood, Stem Cell, and Cancer Research Programme, St Vincent's Centre for Applied Medical Research and Department of Hematology and BM Transplant, St Vincent's Hospital, Level 8, 405 Liverpool Street, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia. Electronic address: t.molloy@amr.org.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Induced pluripotent stem cells (iPSCs) are an invaluable resource for the study of human disease. However, there are no standardized methods for differentiation into hematopoietic cells, and there is a lack of robust, direct comparisons of different methodologies. In the current study we improved a feeder-free, serum-free method for generation of hematopoietic cells from iPSCs, and directly compared this with three other commonly used strategies with respect to efficiency, repeatability, hands-on time, and cost. We also investigated their capability and sensitivity to model genetic hematopoietic disorders in cells derived from Down syndrome and β-thalassemia patients. Of these methods, a multistep monolayer-based method incorporating aryl hydrocarbon receptor hyperactivation ("2D-multistep") was the most efficient, generating significantly higher numbers of CD34

Indexed as

Cell DifferentiationHematopoiesisbeta-ThalassemiaCarbazolesCell CountCells, CulturedDown SyndromeEmbryo, MammalianGlobinsHumansInduced Pluripotent Stem Cells6-formylindolo(3,2-b)carbazoleCarbazolesGlobinscell differentiationdisease modelingDown syndromeerythroid progenitor cellshematopoiesishematopoietic progenitor cellshematopoietic stem cellsinduced pluripotent stem cellspluripotent stem cellsβ-thalassemia

Identifiers

PMID32763163
PMCPMC7486192

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.