Evidence map›Paper›PMID 32762707›Full record

ArticleBMC gastroenterology2020

Patient-individual cancer cell lines and tissue analysis delivers no evidence of sequences from DNA viruses in colorectal cancer cells.

Michael Gock, Marcel Kordt, Stephanie Matschos, Christina S Mullins, Michael Linnebacher

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC gastroenterology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Michael GockDepartment of General Surgery, University of Rostock, Rostock, Germany.
Marcel KordtDepartment of General Surgery, Molecular Oncology and Immunotherapy, University of Rostock, Schillingallee 35, D-18057, Rostock, Germany.
Stephanie MatschosDepartment of General Surgery, Molecular Oncology and Immunotherapy, University of Rostock, Schillingallee 35, D-18057, Rostock, Germany.
Christina S MullinsDepartment of General Surgery, Molecular Oncology and Immunotherapy, University of Rostock, Schillingallee 35, D-18057, Rostock, Germany.
Michael LinnebacherDepartment of General Surgery, Molecular Oncology and Immunotherapy, University of Rostock, Schillingallee 35, D-18057, Rostock, Germany. michael.linnebacher@med.uni-rostock.de.ORCID http://orcid.org/0000-0001-8054-1402
University of Rostock · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSeveral DNA viruses are highly suspicious to have oncogenic effects in humans. This study investigates the presence of potentially oncogenic viruses such as SV40, JCV, BKV and EBV in patient-derived colorectal carcinoma (CRC) cells typifying all molecular subtypes of CRC.

methodsSample material (gDNA and cDNA) of a total of 49 patient-individual CRC cell lines and corresponding primary material from 11 patients, including normal, tumor-derived and metastasis-derived tissue were analyzed for sequences of SV40, JVC, BKV and EBV using endpoint PCR. In addition, the susceptibility of CRC cells to JCV and BKV was examined using a long-term cultivation approach of patient-individual cells in the presence of viruses.

resultsNo virus-specific sequences could be detected in all specimens. Likewise, no morphological changes were observed and no evidence for viral infection or integration could be provided after long term CRC cell cultivation in presence of viral particles.

conclusionsIn summary, the presented data suggest that there is no direct correlation between tumorigenesis and viral load and consequently no evidence for a functional role of the DNA viruses included into this analysis in CRC development.

Indexed as

BK VirusColorectal NeoplasmsJC VirusPolyomavirus InfectionsTumor Virus InfectionsCell LineDNA, ViralDNA VirusesHumansDNA, ViralBK virusColorectal carcinomaEpstein-Barr-virusJC virusOncogenic virusPatient-derived carcinoma cell lines

Identifiers

PMID32762707
PMCPMC7409650
OpenAlexW3047487396

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.