ArticleJournal of clinical laboratory analysis2021
Two simple and inexpensive methods for preparing DNA suitable for digital PCR from a small number of cells in 96-well plates.
Article in Journal of clinical laboratory analysis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 7 citations in OpenAlex.
- Cell-Free DNA-Based Theranostics for Inflammatory Disorders.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- On-Site Dual Detection of AirborneMicroorganisms · 2025Article
- Absolute quantification of rare gene targets in limited samples using crude lysate and ddPCR.Scientific reports · 2025Article
- Uncovering the presence of CVPD disease in citrus varieties of South Sulawesi, Indonesia: A molecular approach.Journal, genetic engineering & biotechnology · 2024Article
- Role of miR-584-5p in Lipopolysaccharide-Stimulated Human Bronchial Epithelial Cell Inflammation and Apoptosis.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Two simple and inexpensive methods for preparing DNA suitable for digital PCR from a small number of cells in 96-well plates.Journal of clinical laboratory analysis · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough DNA of high quality can be easily prepared from cultured cells with commercially available kits, many studies involve a large number of samples which increases the cost drastically. We optimized two simple and inexpensive methods for preparing DNA suitable for digital PCR from a small number of cells directly from wells of 96-well plates.
methodsCells (number: 10
resultsFor 1000 cells from one primary culture and two tumor cell lines, DNA was reproducible and obtained with recovery rate (obtained/expected amount of DNA) in the range of 50%-90% as measured by the fluorometer dyes instrument Qubit. Using 8 out of a total of 10 µL DNA solution for 1000 cells, both conventional PCR and digital PCR were successful. For digital PCR, more than 1600 positive droplets were obtained for DNA from 1000 cells using the Direct PCR
conclusionsTwo methods are efficient, especially the Direct PCR
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Registered trials
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