Evidence map›Paper›PMID 32761270›Full record

ArticleArchives of virology2020

Detection of SARS-CoV-2 by RNAscope

Mariano Carossino, Hon S Ip, Juergen A Richt, Kendra Shultz, Kimberly Harper, Alan T Loynachan, Fabio Del Piero, Udeni B R Balasuriya

Abstract read
In one paragraph

Article in Archives of virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
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  4. Article
  5. Review
  6. Validation of an RNAscope assay for the detection of avian influenza A virus.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Imaging Techniques: Essential Tools for the Study of SARS-CoV-2 Infection.Frontiers in cellular and infection microbiology · 2022
    Review
  16. Article
  17. Experimental re-infected cats do not transmit SARS-CoV-2.Emerging microbes & infections · 2021
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mariano CarossinoLouisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
Hon S IpUnited States Department of the Interior, U. S. Geological Survey, National Wildlife Health Center, 6006, Schroeder Road, Madison, WI, 53711, USA.
Juergen A RichtDepartment of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Kansas State University, K-224B Mosier Hall, Manhattan, KS, 66506, USA.
Kendra ShultzLouisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
Kimberly HarperLouisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
Alan T LoynachanUniversity of Kentucky Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY, 40511, USA.
Fabio Del PieroLouisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
Udeni B R BalasuriyaLouisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA. balasuriya1@lsu.edu.ORCID http://orcid.org/0000-0003-0609-678X

Funding

LSU Health Shreveport (US) PG 002165
6 · The paper itself

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope

Indexed as

AnimalsAntibodies, MonoclonalAntibodies, ViralAntisense Elements (Genetics)BetacoronavirusChlorocebus aethiopsClinical Laboratory TechniquesCoronavirus InfectionsCoronavirus Nucleocapsid ProteinsCOVID-19COVID-19 TestingGenes, ViralHumansImmunohistochemistryIn Situ HybridizationNucleocapsid ProteinsAntibodies, MonoclonalAntibodies, ViralAntisense Elements (Genetics)Coronavirus Nucleocapsid Proteinsnucleocapsid phosphoprotein, SARS-CoV-2Nucleocapsid ProteinsORF1ab polyprotein, SARS-CoV-2PhosphoproteinsPolyproteinsRNA, ViralSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Viral Proteins

Identifiers

PMID32761270
PMCPMC7406679

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.