ArticlePLoS biology2020
Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry.
Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- CrisprBuildr: an open-source application for CRISPR-mediated genome engineering in Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- Asymmetric centromere and gene locus positioning inbioRxiv : the preprint server for biology · 2025Article
- The PCM scaffold enables RNA localization to centrosomes.Molecular biology of the cell · 2025Article
- Asymmetry of centrosomes inMolecular biology of the cell · 2025Article
- Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle.Scientific reports · 2025Article
- Centrosome age breaks spindle size symmetry even in cells thought to divide symmetrically.The Journal of cell biology · 2024Article
- Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains.Journal of visualized experiments : JoVE · 2023Article
- APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells.EMBO reports · 2023Article
- Asymmetric chromatin retention and nuclear envelopes separate chromosomes in fused cells in vivo.Communications biology · 2022Article
- Pericentrin interacts with Kinesin-1 to drive centriole motility.The Journal of cell biology · 2022Article
- Optogenetic approaches for understanding homeostatic and degenerative processes in Drosophila.Cellular and molecular life sciences : CMLS · 2021Review
- Centrosome-centric view of asymmetric stem cell division.Open biology · 2021Review
- PLK-1 Regulation of Asymmetric Cell Division in the EarlyFrontiers in cell and developmental biology · 2020Review
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
Abstract
Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older "mother" and a younger "daughter" centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb's asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo's daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.
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