Evidence map›Paper›PMID 32759734›Full record

ArticleMethods and protocols2020

Tracking Single Cells Motility on Different Substrates.

Pooja Sharma, Van K Lam, Christopher B Raub, Byung Min Chung

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Pooja SharmaDepartment of Biology, The Catholic University of America, Washington, DC 20064, USA.ORCID 0000-0002-6359-5043
Van K LamDepartment of Biomedical Engineering, The Catholic University of America, Washington, DC 20064, USA.
Christopher B RaubDepartment of Biomedical Engineering, The Catholic University of America, Washington, DC 20064, USA.
Byung Min ChungDepartment of Biology, The Catholic University of America, Washington, DC 20064, USA.ORCID 0000-0003-1262-5107

Funding

Mechanism and Impact of an Interaction Between Keratin 19 and a Regulator of Gene ExpressionR15CA213071 · NCI · CATHOLIC UNIVERSITY OF AMERICA · PI CHUNG, BYUNG MIN · 2018 to 2018
$473k
A dual-modality quantitative phase and polarized light microscope to assess cell motility and extracellular matrix remodeling during invasionR03EB028017 · NIBIB · CATHOLIC UNIVERSITY OF AMERICA · PI RAUB, CHRISTOPHER B · 2019 to 2020
$159k
NCI NIH HHS R15 CA213071NIBIB NIH HHS R03 EB028017
6 · The paper itself

Abstract

Motility is a key property of a cell, required for several physiological processes, including embryonic development, axon guidance, tissue regeneration, gastrulation, immune response, and cancer metastasis. Therefore, the ability to examine cell motility, especially at a single cell level, is important for understanding various biological processes. Several different assays are currently available to examine cell motility. However, studying cell motility at a single cell level can be costly and/or challenging. Here, we describe a method of tracking random cell motility on different substrates such as glass, tissue-culture polystyrene, and type I collagen hydrogels, which can be modified to generate different collagen network microstructures. In this study we tracked MDA-MB-231 breast cancer cells using The CytoSMART

Indexed as

collagen extracellular matrixImageJlive cell trackingsingle cell motilitysubstrate stiffnesswrMTrck plugin

Identifiers

PMID32759734
PMCPMC7564475

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.