Trial reportViruses2020
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry.
Trial report in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Detection accuracy and clinical applications of DP-TOF mass spectrometry.The Journal of international medical research · 2024Article
- Performance of MassARRAY system for the detection of SARS-CoV-2 compared to real-time PCR.European journal of microbiology & immunology · 2023Article
- Advancement in COVID-19 detection using nanomaterial-based biosensors.Exploration (Beijing, China) · 2023Review
- Development of a high-resolution mass-spectrometry-based method and software for human leukocyte antigen typing.Frontiers in immunology · 2023Article
- COVID-19 Salivary Protein Profile: Unravelling Molecular Aspects of SARS-CoV-2 Infection.Journal of clinical medicine · 2022Article
- Consolidating the potency of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) in viral diagnosis: Extrapolating its applicability for COVID diagnosis?Trends in analytical chemistry : TRAC · 2022Review
- Article
- Mass spectrometry applied to diagnosis, prognosis, and therapeutic targets identification for the novel coronavirus SARS-CoV-2: A review.Analytica chimica acta · 2022Review
- Visual Detection of COVID-19 from Materials Aspect.Advanced fiber materials · 2022Review
- Coronavirus Disease 2019 (COVID-19): Emerging detection technologies and auxiliary analysis.Journal of clinical laboratory analysis · 2022Review
- Diagnostic Approaches for COVID-19 and Its Associated Complications.Diagnostics (Basel, Switzerland) · 2021Review
- A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.Clinical proteomics · 2021Article
- Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).Pathology, research and practice · 2021Review
- Mass spectrometry analytical responses to the SARS-CoV2 coronavirus in review.Trends in analytical chemistry : TRAC · 2021Review
- Laboratory Diagnosis of SARS-CoV-2 Pneumonia.Diagnostics (Basel, Switzerland) · 2021Review
- Article
- 'The long tail of Covid-19' - The detection of a prolonged inflammatory response after a SARS-CoV-2 infection in asymptomatic and mildly affected patients.F1000Research · 2020Article
- Gastrodin Inhibits Virus Infection by Promoting the Production of Type I Interferon.Frontiers in pharmacology · 2020Article
- Proteomics-based mass spectrometry profiling of SARS-CoV-2 infection from human nasopharyngeal samples.Mass spectrometry reviewsReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAmplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS).
methodsViral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared.
resultsResults of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays.
conclusionsMS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.