ArticleProceedings of the National Academy of Sciences of the United States of America2020
Direct imaging of liquid domains in membranes by cryo-electron tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 92 citations in OpenAlex.
- Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids.Biochimica et biophysica acta. Biomembranes · 2026Article
- Lipids regulate epidermal growth factor receptor activation by its ligands.Biochemical Society transactions · 2026Review
- Nanodomain formation in lipid bilayers II: The influence of mixed-chain saturated lipids.bioRxiv : the preprint server for biology · 2026Article
- Systematic membrane thickness variation across cellular organelles revealed by cryo-ET.The Journal of cell biology · 2026Article
- Hemifusomes and interacting proteolipid nanodroplets mediate multi-vesicular body formation.Nature communications · 2025Article
- Horizontal acquisition of prokaryotic hopanoid biosynthesis reorganizes membrane physiology driving lifestyle innovation in a eukaryote.Nature communications · 2025Article
- The Importance of Bilayer Asymmetry in Biological Membranes: Insights from Model Membranes.Membranes · 2025Review
- Unleashing virus structural biology: Probing protein and membrane intermediates in the dynamic process of membrane fusion.QRB discovery · 2025Review
- Lipid Membrane Leaflets Unzip upon Hybridization with Polymer-Rich Nanodomains.Macromolecules · 2024Article
- Membrane lipid nanodomains modulate HCN pacemaker channels in nociceptor DRG neurons.Nature communications · 2024Article
- Hemifusomes and Interacting Proteolipid Nanodroplets Mediate Multi-Vesicular Body Formation.Research square · 2024Article
- Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios.Biophysical journal · 2024Article
- Visualizing intermediate stages of viral membrane fusion by cryo-electron tomography.Trends in biochemical sciences · 2024Review
- Cryo-EM images of phase-separated lipid bilayer vesicles analyzed with a machine-learning approach.Biophysical journal · 2024Article
- Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Efficient calculation of orientation-dependent lipid dynamics from membrane simulations.bioRxiv : the preprint server for biology · 2024Article
- Experimentally determined leaflet-leaflet phase diagram of an asymmetric lipid bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Direct regulation of the voltage sensor of HCN channels by membrane lipid compartmentalization.Nature communications · 2023Article
- A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization.Biomolecules · 2023Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Images of micrometer-scale domains in lipid bilayers have provided the gold standard of model-free evidence to understand the domains' shapes, sizes, and distributions. Corresponding techniques to directly and quantitatively assess smaller (nanoscale and submicron) liquid domains have been limited. Researchers commonly seek to correlate activities of membrane proteins with attributes of the domains in which they reside; doing so hinges on identification and characterization of membrane domains. Although some features of membrane domains can be probed by indirect methods, these methods are often constrained by the limitation that data must be analyzed in the context of models that require multiple assumptions or parameters. Here, we address this challenge by developing and testing two methods of identifying submicron domains in biomimetic membranes. Both methods leverage cryo-electron tomograms of ternary membranes under vitrified, hydrated conditions. The first method is optimized for probe-free applications: Domains are directly distinguished from the surrounding membrane by their thickness. This technique quantitatively and accurately measures area fractions of domains, in excellent agreement with known phase diagrams. The second method is optimized for applications in which a single label is deployed for imaging membranes by both high-resolution cryo-electron tomography and diffraction-limited optical microscopy. For this method, we test a panel of probes, find that a trimeric mCherry label performs best, and specify criteria for developing future high-performance, dual-use probes. These developments have led to direct and quantitative imaging of submicron membrane domains in vitrified, hydrated vesicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.