ArticleProceedings of the National Academy of Sciences of the United States of America2020
Two distinct pathways of pregranulosa cell differentiation support follicle formation in the mouse ovary.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.
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Who cites it
149 citing papers in PubMed, 293 citations in OpenAlex.
- Molecular mechanisms of folliculogenesis and oogenesis.Systems biology in reproductive medicine · 2026Review
- Standardizing Cell Ontology terms for cross-study integration in the female reproductive tract.Science advances · 2026Review
- Spatiotemporally primed coelomic epithelium and supporting-like cells establish the dual origin of granulosa cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mapping ovarian cellular and molecular landscape across the lifespan of women: a scoping review.Human reproduction update · 2026Article
- Prenatal exposure to polystyrene nanoplastics impairs offspring fertility in mice by disrupting meiotic recombination and chromatin accessibility in oocytes.Archives of toxicology · 2026Article
- The Drosophila ovary produces two waves of adult follicles and a previously unreported pupal wave that turns over.Development (Cambridge, England) · 2026Article
- Perifollicular stromal cells sustain folliculogenesis via MDK-NCL signaling in mice and humans.Cell discovery · 2026Article
- Aging disrupts spatiotemporal coordination in the cycling murine ovary.Nature aging · 2026Article
- Dynamic blebbing and absence of organelle transfer during mouse oocyte formation.The EMBO journal · 2026Article
- Digital Chromosome Banding Reveals Distinct Spatiotemporal Dynamics and Sexual Dimorphism in Meiotic Silencing.bioRxiv : the preprint server for biology · 2026Article
- A Novel BIRC6 Variant Impairs Apoptotic Regulation in Familial Premature Ovarian Insufficiency: Functional Validation in a CRISPR/Cas9 Zebrafish Model.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes.bioRxiv : the preprint server for biology · 2026Article
- Dissecting the transcriptomic signature of porcine oocyte during primordial follicle formation from single cell resolution.Journal of ovarian research · 2026Article
- Gene regulatory networks orchestrating oocyte fate bifurcation in primordial follicles revealed by single-cell transcriptomics.Communications biology · 2026Article
- Polycomb shapes active chromatin and promoter bivalency during ovarian reserve formation and activation.bioRxiv : the preprint server for biology · 2026Article
- Specification of the interstitial cell lineage in the fetal mouse ovary requires balanced Notch signaling.bioRxiv : the preprint server for biology · 2026Article
- A spatiotemporal transcriptomic atlas of porcine (Sus scrofa) female early gonadal development.Communications biology · 2026Article
- Article
- Macrophages regulate meiotic initiation and germ cell clearance in the developing ovary.bioRxiv : the preprint server for biology · 2026Article
- Dietary β-carotene improves the ovary development and antioxidant capacity of replacement gilts.Journal of animal science and biotechnology · 2026Article
89 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We sequenced more than 52,500 single cells from embryonic day 11.5 (E11.5) postembryonic day 5 (P5) gonads and performed lineage tracing to analyze primordial follicles and wave 1 medullar follicles during mouse fetal and perinatal oogenesis. Germ cells clustered into six meiotic substages, as well as dying/nurse cells. Wnt-expressing bipotential precursors already present at E11.5 are followed at each developmental stage by two groups of ovarian pregranulosa (PG) cells. One PG group, bipotential pregranulosa (BPG) cells, derives directly from bipotential precursors, expresses Foxl2 early, and associates with cysts throughout the ovary by E12.5. A second PG group, epithelial pregranulosa (EPG) cells, arises in the ovarian surface epithelium, ingresses cortically by E12.5 or earlier, expresses Lgr5, but delays robust Foxl2 expression until after birth. By E19.5, EPG cells predominate in the cortex and differentiate into granulosa cells of quiescent primordial follicles. In contrast, medullar BPG cells differentiate along a distinct pathway to become wave 1 granulosa cells. Reflecting their separate somatic cellular lineages, second wave follicles were ablated by diptheria toxin treatment of Lgr5-DTR-EGFP mice at E16.5 while first wave follicles developed normally and supported fertility. These studies provide insights into ovarian somatic cells and a resource to study the development, physiology, and evolutionary conservation of mammalian ovarian follicles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.