ArticleThe Science of the total environment2020
Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater.
Article in The Science of the total environment, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 280 papers, 5 of them syntheses that pooled it.
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Who cites it
280 citing papers in PubMed, 5 syntheses or guidelines pooled it, 565 citations in OpenAlex.
- Prevalence of Human Bocavirus in Sewage, Surface Waters, and Other Environmental Milieux: A Meta-regression Modelling.Food and environmental virology · 2025Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- SARS-CoV-2 shedding sources in wastewater and implications for wastewater-based epidemiology.Journal of hazardous materials · 2022Pooled it
- Wastewater surveillance to infer COVID-19 transmission: A systematic review.The Science of the total environment · 2022Pooled it
- Data-driven estimation of COVID-19 community prevalence through wastewater-based epidemiology.The Science of the total environment · 2021Pooled it
- A Wastewater-based Modeling Framework for Inferring Norovirus Transmission Dynamics.Environmental science & technology · 2026Article
- Toward a quality-managed operational architecture for wastewater surveillance of zoonotic and emerging pathogens.Applied and environmental microbiology · 2026Review
- Kinetic Modelling of the Decay of Respiratory Syncytial Viruses (RSV) Facilitated a Robust Wastewater-Based Surveillance for RSV.Food and environmental virology · 2026Article
- Environmental surveillance of pathogens in Africa.Applied and environmental microbiology · 2026Review
- Article
- Comparative Evaluation of DNA Extraction Workflows for Efficient Recovery of pBI143 from Wastewater.Food and environmental virology · 2026Article
- Development and Validation of a Quantitative RT-qPCR Panel for the Detection and Monitoring of Polioviruses in Wastewater Samples.Microorganisms · 2026Article
- Effect of nucleic acid concentration method on Severe Acute Respiratory Syndrome Coronavirus 2 detection sensitivity in aircraft wastewater.Journal of medical microbiology · 2026Article
- SARS-CoV-2 wastewater genomic surveillance: approaches, challenges, and opportunities.Genome biology · 2026Review
- SARS-CoV-2 Surface Contamination and Viable Virus in High-Ventilation Single Rooms: Implications for Fomite Transmission.Juntendo medical journal · 2026Article
- Comparative virome analysis of lake and domestic wastewater revealed the unexpected presence of swine acute diarrhea syndrome coronavirus andFrontiers in microbiology · 2026Article
- Wastewater-based surveillance of microbial pathogens in GCC countries (2015-2025): a scoping review and questionnaire survey with stakeholders.Frontiers in public health · 2026Article
- Early-Stage Adhesion Dynamics of Viral Hemorrhagic Septicemia Virus in Olive Flounder, Paralichthys olivaceus.Journal of fish diseases · 2025Article
- Seasonal patterns of viromes in urban aquatic environments of Manitoba.Applied and environmental microbiology · 2025Article
- Relating antimicrobial use to wastewater resistance gene patterns via metagenomic analysis of two neighboring treatment plants circa the COVID-19 pandemic.npj antimicrobials and resistance · 2025Article
220 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 11 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is currently a clear benefit for many countries to utilize wastewater-based epidemiology (WBE) as part of ongoing measures to manage the coronavirus disease 2019 (COVID-19) global pandemic. Since most wastewater virus concentration methods were developed and validated for nonenveloped viruses, it is imperative to determine the efficiency of the most commonly used methods for the enveloped severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Municipal wastewater seeded with a human coronavirus (CoV) surrogate, murine hepatitis virus (MHV), was used to test the efficiency of seven wastewater virus concentration methods: (A-C) adsorption-extraction with three different pre-treatment options, (D-E) centrifugal filter device methods with two different devices, (F) polyethylene glycol (PEG 8000) precipitation, and (G) ultracentrifugation. MHV was quantified by reverse-transcription quantitative polymerase chain reaction and the recovery efficiency was calculated for each method. The mean MHV recoveries ranged from 26.7 to 65.7%. The most efficient methods were adsorption-extraction methods with MgCl
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.