ArticleNeuropsychopharmacology reports2020
A live imaging-friendly slice culture method using collagen membranes.
Article in Neuropsychopharmacology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 4 citations in OpenAlex.
- Live-Cell Imaging of Microglia in Organotypic Brain Slices Using Microcontact Printing.Biomolecules · 2026Article
- Ultra-thin and ultra-porous nanofiber networks as a basement-membrane mimic.Lab on a chip · 2023Article
- Replacement of Mouse Microglia With Human Induced Pluripotent Stem Cell (hiPSC)-Derived Microglia in Mouse Organotypic Slice Cultures.Frontiers in cellular neuroscience · 2022Article
- Assessing Microglial Dynamics by Live Imaging.Frontiers in immunology · 2021Review
- A live imaging-friendly slice culture method using collagen membranes.Neuropsychopharmacology reports · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
aimOrganotypic brain slice culture preserves the geographical position of neurons and neuronal circuits. The slice cultures also maintain both non-neuronal cell types and the surrounding extracellular matrix. The interface method has been widely used for slice cultures, in which brain slices are placed on semiporous polytetrafluoroethylene (PTFE) membranes. However, a low optical transparency of PTFE membrane makes it difficult to perform live imaging of deep regions of slice cultures using an inverted microscope. To overcome the issue, we evaluated the suitability of using collagen membranes for slice cultures, especially focusing on live imaging of the cellular dynamics of green fluorescent protein (GFP)-expressing microglia.
methodsEntorhinohippocampal slices were cultured on either collagen or PTFE membranes. The influence of membrane type on the ability to observe deep regions of slice cultures was examined by live imaging using an inverted microscope.
resultsCollagen membranes were thinner and had better optical transparency compared with PTFE membranes. There were no differences in cell viability, density of neurons or microglia. The densify of visible short branches of microglia in live imaging was higher in collagen membranes than PTFE membranes.
conclusionCollagen membranes are suitable for live imaging of cellular dynamics in slice cultures using an inverted microscope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.