Evidence map›Paper›PMID 32757070›Full record

ArticleMikrochimica acta2020

Cross-linkage urease nanoparticles: a high-efficiency signal-generation tag for portable pH meter-based electrochemical immunoassay of lipocalin-2 protein diagnostics.

Ai-Li Sun, Qing-An Qi, Li-Juan Zhi

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Technologies for Frugal and Sensitive Point-of-Care Immunoassays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Ai-Li SunKey Laboratory of Nano-carbon Modified Film Technology Engineering (Henan Province), Department of Pharmacy, Xinxiang University, Xinxiang, 453000, China.
Qing-An QiDepartment of General Surgery, The Hospital of Eighty-Third Army, Xinxiang, 453000, Henan, China. qiqingan@126.com.
Li-Juan ZhiDepartment of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang, 453000, Henan, China.
Xinxiang University · CN

Funding

National Natural Science Foundation of China 51802275
6 · The paper itself

Abstract

An innovative signal-transduction tag based on cross-linked urease nanoparticles (CLENP) was designed for the development of a pH meter-based immunoassay of lipocalin-2 (LCN2). The CLENP was synthesized with a typical desolvation method using ethanol as desolvation agent, followed by functionalization with polyaspartic acid. The carboxylated CLENP were used as the signal-generation tags for the labelling of secondary antibodies via the carbodiimide coupling. Upon target LCN2 introduction, a sandwich-type immune reaction was performed between capture antibody-coated plate and the labeled secondary antibody on the CLENP. The conjugated CLENP in the microplate hydrolyzed urea into ammonia (NH

Indexed as

Antibodies, ImmobilizedAntibodies, MonoclonalCanavaliaElectrochemical TechniquesEnzymes, ImmobilizedHumansHydrogen-Ion ConcentrationImmunoassayLimit of DetectionLipocalin-2NanoparticlesProof of Concept StudyUreaUreaseAntibodies, ImmobilizedAntibodies, MonoclonalEnzymes, ImmobilizedLCN2 protein, humanLipocalin-2UreaUreaseCross-linkage urease nanoparticlesElectrochemical immunoassayEnzymatic hydrolysispH measurementSignal-transduction tags

Identifiers

PMID32757070
OpenAlexW3047233684

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.