ArticleThe Journal of antimicrobial chemotherapy2020
A genome-wide analysis of Escherichia coli responses to fosfomycin using TraDIS-Xpress reveals novel roles for phosphonate degradation and phosphate transport systems.
Article in The Journal of antimicrobial chemotherapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Mapping the genomic landscape of antibiotic susceptiblity in Escherichia coli.npj antimicrobials and resistance · 2026Article
- The Metabolic State ofACS infectious diseases · 2026Article
- Metabolic fingerprinting to elucidate the biodegradation of phosphonoacetic acid and its impact on Penicillium metabolism.Biodegradation · 2025Article
- Tazobactam selects for multidrug resistance.npj antimicrobials and resistance · 2025Article
- Metabolic state-driven nutrient-based approach to combat bacterial antibiotic resistance.npj antimicrobials and resistance · 2025Review
- Chromosomal genes modulating fosfomycin susceptibility in uropathogenicAntimicrobial agents and chemotherapy · 2025Article
- Genetic requirements for uropathogenicmSystems · 2024Article
- Genome-wide identification of fitness-genes in aminoglycoside-resistant Escherichia coli during antibiotic stress.Scientific reports · 2024Article
- Inactivation ofMicrobiology spectrum · 2023Article
- Genome-wide analysis of genes involved in efflux function and regulation withinMicrobiology (Reading, England) · 2023Article
- Long-read sequencing for identification of insertion sites in large transposon mutant libraries.Scientific reports · 2022Article
- Loss of YhcB results in dysregulation of coordinated peptidoglycan, LPS and phospholipid synthesis during Escherichia coli cell growth.PLoS genetics · 2021Article
- Chemical biology-whole genome engineering datasets predict new antibacterial combinations.Microbial genomics · 2021Article
- The Transporter-Mediated Cellular Uptake and Efflux of Pharmaceutical Drugs and Biotechnology Products: How and Why Phospholipid Bilayer Transport Is Negligible in Real Biomembranes.Molecules (Basel, Switzerland) · 2021Review
- High-density transposon libraries utilising outward-oriented promoters identify mechanisms of action and resistance to antimicrobials.FEMS microbiology letters · 2020Article
- Detection of Low-Level Fosfomycin-Resistant Variants by Decreasing Glucose-6-Phosphate Concentration in Fosfomycin Susceptibility Determination.Antibiotics (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundFosfomycin is an antibiotic that has seen a revival in use due to its unique mechanism of action and efficacy against isolates resistant to many other antibiotics. In Escherichia coli, fosfomycin often selects for loss-of-function mutations within the genes encoding the sugar importers, GlpT and UhpT. There has, however, not been a genome-wide analysis of the basis for fosfomycin susceptibility reported to date.
methodsHere we used TraDIS-Xpress, a high-density transposon mutagenesis approach, to assay the role of all genes in E. coli involved in fosfomycin susceptibility.
resultsThe data confirmed known fosfomycin susceptibility mechanisms and identified new ones. The assay was able to identify domains within proteins of importance and revealed essential genes with roles in fosfomycin susceptibility based on expression changes. Novel mechanisms of fosfomycin susceptibility that were identified included those involved in glucose metabolism and phosphonate catabolism (phnC-M), and the phosphate importer, PstSACB. The impact of these genes on fosfomycin susceptibility was validated by measuring the susceptibility of defined inactivation mutants.
conclusionsThis work reveals a wider set of genes that contribute to fosfomycin susceptibility, including core sugar metabolism genes and two systems involved in phosphate uptake and metabolism previously unrecognized as having a role in fosfomycin susceptibility.
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