Evidence map›Paper›PMID 32756532›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2020

Exosomes Mediated Transfer of Circ_UBE2D2 Enhances the Resistance of Breast Cancer to Tamoxifen by Binding to MiR-200a-3p.

Ke Hu, Xianhao Liu, Yi Li, Qinyang Li, Yijun Xu, Wei Zeng, Guocheng Zhong, Changhua Yu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
55citing papers in PubMed, 1 pooled it
4.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

55 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Ke HuDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Xianhao LiuDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Yi LiDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Qinyang LiDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Yijun XuDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Wei ZengDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Guocheng ZhongDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Changhua YuDepartment of Hematology and Oncology, Shenzhen University General Hospital (Shenzhen University Clinical Medical Academy), Shenzhen, Guangdong, China (mainland).
Shenzhen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND Circular RNA UBE2D2 (circ_UBE2D2) has been found to be involved in the progression of breast cancer. Exosomes are critical mediators of intercellular communication, however, the function of exosomal circ_UBE2D2 in breast cancer remains vague. MATERIAL AND METHODS Cell viability was measured by Cell Counting Kit-8 assay. Western blot was used to detect the levels of estrogen receptor alpha (ERalpha), E-cadherin, vimentin, CD9, and CD63. Migrated and invaded cells were examined using Transwell assay. Circ_UBE2D2 and microRNA (miR)-200a-3p levels were detected using quantitative real-time polymerase chain reaction. Exosomes were isolated by ultracentrifugation method. The interaction between circ_UBE2D2 and miR-200a-3p was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Murine xenograft model was established to conduct in vivo experiments. RESULTS We found that circ_UBE2D2 was upregulated in breast cancer tamoxifen-resistant tissues and cell lines, and circ_UBE2D2 deletion mitigated tamoxifen resistance in breast cancer cells. Circ_UBE2D2 was also significantly loaded in exosomes isolated from resistant cells and could be transferred to parental cells. MiR-200a-3p was a target of circ_UBE2D2, and we demonstrated that exosomes mediated transfer of circ_UBE2D2 interacted with miR-200a-3p to enhance tamoxifen resistance of breast cancer cells by regulating cell viability, metastasis, and the level of ERalpha in vivo and in vitro. CONCLUSIONS Exosomes mediated transfer of circ_UBE2D2 reinforced tamoxifen resistance in breast cancer by binding to miR-200a-3p, providing new insights into the boost of the effectiveness of tamoxifen on breast cancer patients.

Indexed as

AnimalsBreast NeoplasmsCell MovementCell SurvivalDrug Resistance, NeoplasmEstrogen AntagonistsEstrogen Receptor alphaExosomesFemaleHEK293 CellsHumansMCF-7 CellsMiceMice, Inbred BALB CMice, NudeMicroRNAsESR1 protein, humanEstrogen AntagonistsEstrogen Receptor alphaMicroRNAsMIRN200 microRNA, humanRNA, CircularTamoxifenUBE2D2 protein, humanUbiquitin-Conjugating Enzymes

Identifiers

PMID32756532
PMCPMC7431386
OpenAlexW3034269777

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.