ArticleMethods (San Diego, Calif.)2021
Targeted bisulfite sequencing for biomarker discovery.
Article in Methods (San Diego, Calif.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Circulating cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) in oncology: from technological advances to clinical applications.NPJ precision oncology · 2026Review
- DNA methylation profiles at hospital admission are associated with subsequent severe COVID-19 outcomes.Clinical epigenetics · 2026Article
- Epigenetic profiling of circulating cell-free DNA for early detection and minimal residual disease assessment in lung cancer: a focus on DNA methylation.Frontiers in oncology · 2026Review
- The technology landscape for detection of DNA methylation in cancer liquid biopsies.Epigenetics · 2025Review
- Epigenetic profiles of tissue informative CpGs inform ALS disease status and progression.Genome medicine · 2025Article
- Frailty in lung transplant recipients is associated with anemia and telomere dysfunction but independent of epigenetic age.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2025Article
- Cost-effective promoter methylation analysis via target long-read bisulfite sequencing: a case study in severe preterm birth.BMC medical genomics · 2025Article
- DNA methylation predicts infection risk in kidney transplant recipients.Life science alliance · 2025Article
- An epigenetic clock for Xenopus tropicalis.npj aging · 2025Article
- Article
- Polymerase Chain Reaction Chips for Biomarker Discovery and Validation in Drug Development.Micromachines · 2025Review
- Epigenetic aging of semen is associated with inflammation.Epigenetics · 2024Article
- An epigenetic human cytomegalovirus infection score predicts viremia risk in seropositive lung transplant recipients.Epigenetics · 2024Article
- The response to influenza vaccination is associated with DNA methylation-driven regulation of T cell innate antiviral pathways.Clinical epigenetics · 2024Article
- Correlation of ChREBP Gene Methylation with Pathological Characteristics of Type 2 Diabetes Mellitus.Applied biochemistry and biotechnology · 2024Article
- The response to influenza vaccination is associated with DNA methylation-driven regulation of T cell innate antiviral pathways.Research square · 2024Article
- Tissue informative cell-free DNA methylation sites in amyotrophic lateral sclerosis.medRxiv : the preprint server for health sciences · 2024Article
- Prediction of methylation status using WGS data of plasma cfDNA for multi-cancer early detection (MCED).Clinical epigenetics · 2024Article
- Longitudinal analysis of influenza vaccination implicates regulation of RIG-I signaling by DNA methylation.Scientific reports · 2024Article
- Epigenetic changes to gene pathways linked to male fertility in ex situ black-footed ferrets.Evolutionary applications · 2024Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.